Kivela A J, Saarnio J, Karttunen T J, Kivelä J, Parkkila A K, Pastorekova S, Pastorek J, Waheed A, Sly W S, Parkkila T S, Rajaniemi H
Department of Anatomy and Cell Biology, University of Oulu, Finland.
Dig Dis Sci. 2001 Oct;46(10):2179-86. doi: 10.1023/a:1011910931210.
This study compares the localization of carbonic anhydrase isozymes (CA) I and II and that of IX and XII in normal large intestine and in colorectal tumors. Immunohistochemical studies were performed on 69 colorectal lesions. While the normal mucosa of the large intestine showed high expression for CA I and II, the intensity of the immunostaining for both isozymes decreased in benign lesions and was very weak in malignant tumors. The reciprocal pattern of expression observed for these cytoplasmic isozymes and transmembrane CA IX and XII in intestinal tissue specimens supports the suggestion that CA IX and XII may be functionally involved in tumor progression to malignancy and/or in invasion. By contrast, while CA I and II are prominent in normal colorectal mucosa, where they play a role in regulation of pH homeostasis and water and ion transport, loss of expression of these cytoplasmic isozymes consistently accompanies progression to malignant transformation.
本研究比较了碳酸酐酶同工酶（CA）I和II以及IX和XII在正常大肠和结直肠癌中的定位。对69个结直肠病变进行了免疫组织化学研究。大肠正常黏膜显示CA I和II高表达，而在良性病变中这两种同工酶的免疫染色强度降低，在恶性肿瘤中则非常弱。在肠道组织标本中观察到这些细胞质同工酶与跨膜CA IX和XII的表达模式相反，这支持了CA IX和XII可能在肿瘤进展为恶性和/或侵袭过程中发挥功能作用的观点。相比之下，虽然CA I和II在正常结直肠黏膜中很突出，它们在调节pH稳态以及水和离子转运中发挥作用，但这些细胞质同工酶表达的丧失始终伴随着向恶性转化的进展。
