病毒和真核生物RNA中聚腺苷酸序列的检测（聚（U）纤维素柱 - 聚（U）滤膜 - 玻璃纤维 - 海拉细胞 - 噬菌体T4）
Detection of polyadenylic acid sequences in viral and eukaryotic RNA(polu(U)-cellulose columns-poly(U) filters-fiberglass-HeLa cells-bacteriophage T4).
作者信息
Sheldon R, Jurale C, Kates J
出版信息
Proc Natl Acad Sci U S A. 1972 Feb;69(2):417-21. doi: 10.1073/pnas.69.2.417.
Abstract
A rapid and specific technique to detect polyriboadenylic acid sequences in RNA is described. The method depends upon the ability of RNAs that contain poly(A) sequences to associate specifically with poly(U) that has been immobilized on fiberglass filters by ultraviolet irradiation. A high proportion of the transcripts synthesized in vivo and in vitro from the vaccinia virus genome contain poly(A) sequences and bind to the poly(U) filters. Similarly, DNA-like RNA from the nucleus and from the cytoplasmic polyribosomes of HeLa cells is rich in species that bind to poly(U) filters. Poly(U) immobilized on cellulose powder is useful to make columns with a high capacity for the binding and purification of poly(A)-containing RNAs.
摘要
本文描述了一种快速且特异的检测RNA中多聚腺苷酸序列的技术。该方法基于含有多聚（A）序列的RNA能够与通过紫外线照射固定在玻璃纤维滤膜上的多聚（U）特异性结合的能力。从痘苗病毒基因组在体内和体外合成的转录本中，很大一部分含有多聚（A）序列并能与多聚（U）滤膜结合。同样，来自HeLa细胞核和细胞质多核糖体的类DNA RNA富含能与多聚（U）滤膜结合的种类。固定在纤维素粉末上的多聚（U）可用于制备具有高容量结合和纯化含多聚（A）RNA的柱子。
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1
Detection of polyadenylic acid sequences in viral and eukaryotic RNA(polu(U)-cellulose columns-poly(U) filters-fiberglass-HeLa cells-bacteriophage T4).病毒和真核生物RNA中聚腺苷酸序列的检测（聚（U）纤维素柱 - 聚（U）滤膜 - 玻璃纤维 - 海拉细胞 - 噬菌体T4）
Proc Natl Acad Sci U S A. 1972 Feb;69(2):417-21. doi: 10.1073/pnas.69.2.417.
2
Adsorption of polyadenylate and other polynucleotides to unmodified cellulose.聚腺苷酸及其他多核苷酸对未修饰纤维素的吸附作用。
Biochemistry. 1973 Dec 4;12(25):5086-91. doi: 10.1021/bi00749a010.
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The genome of RNA tumor viruses contains polyadenylic acid sequences.RNA肿瘤病毒的基因组含有聚腺苷酸序列。
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4
Polyadenylic acid on poliovirus RNA. III. In vitro addition of polyadenylic acid to poliovirus RNAs.脊髓灰质炎病毒核糖核酸上的聚腺苷酸。III. 体外将聚腺苷酸添加到脊髓灰质炎病毒核糖核酸上。
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Biochemistry. 1974 Aug 27;13(18):3677-82. doi: 10.1021/bi00715a009.
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In vitro synthesis of RNA that contains polyadenylate by virion-associated RNA polymerase of vesicular stomatitis virus.水泡性口炎病毒的病毒粒子相关RNA聚合酶在体外合成含聚腺苷酸的RNA。
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Mechanism of poly(A) synthesis by vaccinia virus.痘苗病毒合成多聚腺苷酸的机制。
J Virol. 1974 Aug;14(2):214-24. doi: 10.1128/JVI.14.2.214-224.1974.
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Messenger RNA synthesis by a "coated" viral genome.由“包膜”病毒基因组进行信使核糖核酸合成。
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Polyadenylic acid in the cytoplasm of rat liver.大鼠肝脏细胞质中的聚腺苷酸。
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A strand disproportionation reaction in a helical polynucleotide system.螺旋多核苷酸系统中的链歧化反应。
Biochem Biophys Res Commun. 1964;14:129-36. doi: 10.1016/0006-291x(64)90242-6.
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Metabolism of naturally occurring polymers of adenylic acid and guanylic acid.腺苷酸和鸟苷酸天然聚合物的代谢。
Biochem Biophys Res Commun. 1969 Feb 21;34(4):536-40. doi: 10.1016/0006-291x(69)90415-x.
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The isolation and characterization of adenosine monophosphate-rich polynucleotides synthesized by Ehrlich ascites cells.由艾氏腹水癌细胞合成的富含单磷酸腺苷的多核苷酸的分离与特性研究。
J Biol Chem. 1969 Mar 10;244(5):1314-24.
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