Wehner R, Handke A
J Chromatogr. 1979 Sep 21;177(2):237-44. doi: 10.1016/s0021-9673(01)96319-8.
A method for estimating progesterone in rat plasma using gas-liquid chromatography (GLC) with electron-capture detection is described. By means of a column chromatographic technique for the extraction of progesterone from plasma, it is possible to isolate the steroid in such a specific way that, contrary to previously published methods, no additional chromatographic purification and separation steps are needed prior to the final GLC analysis. As a derivative for electron-capture detection we used the 3,20-di-O-pentafluorobenzyloxime of progesterone. This derivative is superior to those used hitherto with regard to sensitivity and stability. The high overall recovery (mean value, 89.4%) makes this method especially convenient for research work when only little plasma is available, or when the concentration of progesterone is very low.
描述了一种使用带电子捕获检测的气液色谱法（GLC）估算大鼠血浆中孕酮的方法。通过柱色谱技术从血浆中提取孕酮，能够以一种特定方式分离该类固醇，与先前发表的方法不同，在最终的GLC分析之前无需额外的色谱纯化和分离步骤。作为电子捕获检测的衍生物，我们使用了孕酮的3,20-二-O-五氟苄基肟。该衍生物在灵敏度和稳定性方面优于迄今使用的那些衍生物。高总体回收率（平均值为89.4%）使得该方法在仅有少量血浆可用或孕酮浓度非常低时，对于研究工作特别方便。
