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A new approach to the synthesis of the 5'-end substituted oligonucleotides using T4 polynucleotide kinase and gamma-amides of ATP bearing photoreactive groups.

作者信息

Petrousseva I O, Safronov I V, Komarova N I, Kamynina T P, Lavrik O I, Khodyreva S N

机构信息

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, pr. Lavrent'eva 8, Novosibirsk, 630090 Russia.

出版信息

Dokl Biochem Biophys. 2003 Mar-Apr;389:114-7. doi: 10.1023/a:1023692324262.

DOI:10.1023/a:1023692324262
PMID:12856418
Abstract
摘要

相似文献

1
A new approach to the synthesis of the 5'-end substituted oligonucleotides using T4 polynucleotide kinase and gamma-amides of ATP bearing photoreactive groups.一种使用T4多核苷酸激酶和带有光反应基团的ATPγ-酰胺合成5'-末端取代寡核苷酸的新方法。
Dokl Biochem Biophys. 2003 Mar-Apr;389:114-7. doi: 10.1023/a:1023692324262.
2
5'-end labeling of RNA with [γ-32P]ATP and T4 polynucleotide kinase.使用[γ-32P]ATP和T4多核苷酸激酶对RNA进行5'末端标记。
Cold Spring Harb Protoc. 2014 Apr 1;2014(4):441-3. doi: 10.1101/pdb.prot080739.
3
Quantification of DNA probes on nylon microarrays using T4 polynucleotide kinase labeling.使用T4多核苷酸激酶标记法对尼龙微阵列上的DNA探针进行定量分析。
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4
Efficient 5'-end labeling of oligonucleotides containing self-complementary sequences.含自身互补序列的寡核苷酸的高效5'末端标记
Biotechniques. 1997 Oct;23(4):658-60. doi: 10.2144/97234bm25.
5
In situ monitoring of a trace intermediate during DNA phosphorylation by T4 polynucleotide kinase for transient kinetic studies.在 T4 多核苷酸激酶进行 DNA 磷酸化过程中痕量中间产物的原位监测，用于瞬态动力学研究。
Analyst. 2012 Mar 21;137(6):1334-7. doi: 10.1039/c2an16273c. Epub 2012 Feb 2.
6
The stereochemical course of phosphoryl transfer catalysed by polynucleotide kinase (bacteriophage-T4-infected Escherichia coli B).多核苷酸激酶（噬菌体T4感染的大肠杆菌B）催化的磷酰基转移的立体化学过程。
Biochem J. 1981 Oct 1;199(1):273-6. doi: 10.1042/bj1990273.
7
Labeling efficiency of oligonucleotides by T4 polynucleotide kinase depends on 5'-nucleotide.T4多核苷酸激酶对寡核苷酸的标记效率取决于5'-核苷酸。
Anal Biochem. 1998 Dec 15;265(2):386-9. doi: 10.1006/abio.1998.2900.
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Thioflavin T as a fluorescence probe for label-free detection of T4 polynucleotide kinase/phosphatase and its inhibitors.硫黄素T作为一种用于无标记检测T4多核苷酸激酶/磷酸酶及其抑制剂的荧光探针。
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Polynucleotide kinase: a versatile molecule makes a clean break.多核苷酸激酶：一种多功能分子实现精准切割。
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A mutation in the gene for polynucleotide kinase of bacteriophage T4 K10 affects mRNA processing.噬菌体 T4 K10 的多核苷酸激酶基因的突变影响 mRNA 加工。
Arch Virol. 2014 Feb;159(2):327-31. doi: 10.1007/s00705-013-1800-x. Epub 2013 Aug 15.

本文引用的文献

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[Interaction of human replication protein A with DNA-duplexes, containing gaps of varying sizes].[人类复制蛋白A与含有不同大小缺口的DNA双链体的相互作用]
Mol Biol (Mosk). 2001 Sep-Oct;35(5):827-35.
2
Affinity labeling of flap-endonuclease FEN-1 by photoreactive DNAs.光反应性DNA对瓣内切核酸酶FEN-1的亲和标记
Biochemistry (Mosc). 2001 Jul;66(7):733-9. doi: 10.1023/a:1010204527130.
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Localization of the large subunit of replication factor C near the 5' end of DNA primers.复制因子C大亚基在DNA引物5'端附近的定位。
J Mol Recognit. 2001 Jul-Aug;14(4):239-44. doi: 10.1002/jmr.538.
4
[Preparation of photoreactive oligonucleotide duplexes and their application for photoaffinity modification of DNA-binding proteins].[光反应性寡核苷酸双链体的制备及其在DNA结合蛋白光亲和修饰中的应用]
Bioorg Khim. 2001 May-Jun;27(3):205-9. doi: 10.1023/a:1011381304702.
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[Reagents for modification of protein-nucleic acids complexes. II. Site-specific photomodification of DNA-polymerase beta complexes with primers elongated by the dCTP exo-N-substituted arylazido derivatives].
Bioorg Khim. 2001 May-Jun;27(3):197-204. doi: 10.1023/a:1011329220632.
6
Polarity of human replication protein A binding to DNA.人类复制蛋白A与DNA结合的极性
Nucleic Acids Res. 2001 Jan 15;29(2):373-9. doi: 10.1093/nar/29.2.373.
7
Replication protein A (RPA): the eukaryotic SSB.复制蛋白A（RPA）：真核单链结合蛋白
Crit Rev Biochem Mol Biol. 1999;34(3):141-80. doi: 10.1080/10409239991209255.
8
The FEN-1 family of structure-specific nucleases in eukaryotic DNA replication, recombination and repair.真核生物DNA复制、重组和修复中结构特异性核酸酶的FEN-1家族。
Bioessays. 1997 Mar;19(3):233-40. doi: 10.1002/bies.950190309.
9
Gamma-phosphate-substituted 2'-deoxynucleoside 5'-triphosphates as substrates for DNA polymerases.γ-磷酸取代的2'-脱氧核苷5'-三磷酸作为DNA聚合酶的底物
J Biol Chem. 1996 Oct 4;271(40):24389-94. doi: 10.1074/jbc.271.40.24389.
10
Enzymatic transfer of ATP gamma S thiophosphate onto the 5'-hydroxyl of an oligonucleotide as a route to reactive oligonucleotide derivatives.通过将三磷酸腺苷γ-硫代磷酸酯（ATPγS）的硫代磷酸基团酶促转移至寡核苷酸的5'-羟基上，来制备反应性寡核苷酸衍生物。
FEBS Lett. 1982 Jun 21;143(1):119-23. doi: 10.1016/0014-5793(82)80286-x.