多核苷酸激酶（噬菌体T4感染的大肠杆菌B）催化的磷酰基转移的立体化学过程。
The stereochemical course of phosphoryl transfer catalysed by polynucleotide kinase (bacteriophage-T4-infected Escherichia coli B).
作者信息
Jarvest R L, Lowe G
出版信息
Biochem J. 1981 Oct 1;199(1):273-6. doi: 10.1042/bj1990273.
Abstract
Polynucleotide kinase (bacteriophage-T4-infected Escherichia coli B) catalyses the transfer of the [gamma-16O,17O,18O]phosphoryl group from 5'[gamma(S)-16O,17O,18O]ATP to 3'-AMP with inversion of configuration at the phosphorus atom. The simplest interpretation of this observation is that the [gamma-16O,17O,18O]phosphoryl group is transferred directly from ATP to the co-substrate by an 'in-line' mechanism.
摘要
多核苷酸激酶（噬菌体T4感染的大肠杆菌B）催化[γ-16O,17O,18O]磷酰基从5'-[γ(S)-16O,17O,18O]ATP转移至3'-AMP，磷原子构型发生翻转。对该观察结果最简单的解释是，[γ-16O,17O,18O]磷酰基通过“直线型”机制直接从ATP转移至共底物。
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