Ma Yu, Xia Yun, Yang Xiaolan, Yu Ming'an
Department of Chemistry, College of Basic Medical Sciences, Chongqing University of Medical Sciences, Chongqing 400016.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2003 Jun;20(2):311-3.
Virus inactivation of plasma can be achieved by phototreatment with methylene blue (MB). Subsequently, elimination of MB may reduce the adverse effects of MB. This study examined the effects of adsorbing MB with the use of cross-linked agar bead entrapped attapulgite clay (CAA) on normal ingredients in MB-treated plasma units. The biomedical characteristics of CAA were assessed by determination of partial biochemical indexes, coagulation potency and some cationic concentration in a control sample and the MB-treated plasma eluted from CAA column. The biochemistry indexes or K+, Na+ in plasma were almost unaltered before and after CAA adsorption. In contrast, the concentrations of CA2+ and Mg2+ increased and the blood ammonium decreased obviously. The activated partial thromboplastin time (APTT) was prolonged from 42 s to 53 s, and prothrombin time (PT) from 13 s to 14 s. The result indicates that CAA as an adsorbent for hemopurification retains the most important characters of human plasma. CAA can be useful for the elimination of MB in MB-treated plasma and does not bring on harmful alteration in clinical significance.
用亚甲蓝（MB）进行光处理可实现血浆的病毒灭活。随后，去除MB可降低其不良反应。本研究考察了使用交联琼脂珠包埋凹凸棒土（CAA）吸附MB对经MB处理的血浆单位中正常成分的影响。通过测定对照样品以及从CAA柱洗脱的经MB处理的血浆中的部分生化指标、凝血能力和一些阳离子浓度，评估了CAA的生物医学特性。CAA吸附前后，血浆中的生化指标或K⁺、Na⁺几乎未发生变化。相比之下，Ca²⁺和Mg²⁺的浓度升高，血氨明显降低。活化部分凝血活酶时间（APTT）从42秒延长至53秒，凝血酶原时间（PT）从13秒延长至14秒。结果表明，CAA作为血液净化吸附剂保留了人血浆的最重要特性。CAA可用于去除经MB处理的血浆中的MB，且不会在临床意义上引起有害改变。
