Lacey Jackie A, Phillips David
Department of Chemistry, Imperial College of Science, Technology and Medicine, London, UK.
Photochem Photobiol Sci. 2002 Jun;1(6):378-83. doi: 10.1039/b108831a.
The fluorescence lifetimes of disulfonated aluminium phthalocyanine (AlPcS2) in the presence of several different microbial cells are measured using the technique of time correlated single photon counting (TCSPC) employing front-face illumination. The microbes studied are: Escherichia coli, Porphyromonas gingivalis (Gram-negative bacteria), Streptococcus mutans (a Gram-positive bacterium), and the yeast Candida albicans. Complex fluorescence decays are observed when AlPcS2 is in the presence of these microbes; the fluorescence decay data can be fitted to a distribution of exponential lifetimes indicating that the AlPcS2 molecules experience a range of micro-environments The average fluorescence lifetimes of AlPcS2 in the presence of the microbes studied range from 4.85 to 5.95 ns, indicating differences in the cellular localisation of AlPcS2 with each of the microbes studied. These novel data are presented alongside a brief summary of existing AlPcS2 fluorescence lifetime data measured both in solution and in model biological systems.
采用前表面照明的时间相关单光子计数（TCSPC）技术，测量了在几种不同微生物细胞存在下二磺酸铝酞菁（AlPcS2）的荧光寿命。所研究的微生物有：大肠杆菌、牙龈卟啉单胞菌（革兰氏阴性菌）、变形链球菌（革兰氏阳性菌）和白色念珠菌。当AlPcS2存在于这些微生物中时，观察到复杂的荧光衰减；荧光衰减数据可以拟合为指数寿命分布，这表明AlPcS2分子经历了一系列微环境。在所研究的微生物存在下，AlPcS2的平均荧光寿命范围为4.85至5.95纳秒，这表明AlPcS2在每种所研究的微生物中的细胞定位存在差异。这些新数据与在溶液和模型生物系统中测量的现有AlPcS2荧光寿命数据的简要总结一起呈现。
