Petrov Raina, MacDonald Melinda H, Tesch Anthony M, Van Hoogmoed Linda M
Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Am J Vet Res. 2003 Jul;64(7):835-44. doi: 10.2460/ajvr.2003.64.835.
To determine rate and degree of cooling for the superficial digital flexor tendon (SDFT) during a standard cryotherapy application in horses and evaluate in vitro effects of cooling on survival of tendon cells.
6 limbs of 5 adult horses and cultured cells obtained from SDFT of 3 adult horses during necropsy.
In vivo data were acquired by use of a thermocouple temperature probe inserted into the SDFT of a forelimb of each standing sedated horse. After baseline temperatures were recorded, a commercial compression splint with circulating coolant was placed on each selected limb, which was then exposed to cold treatment for 60 minutes. Temperatures were recorded at 30-second intervals. Mean minimum core temperature was calculated and used to design a protocol for in vitro cold treatment of cells. Specimens were obtained from the SDFT of horses during necropsy; tendon cells were cultured in suspension and exposed to 1-hour of cold treatment that mimicked the in vivo procedure. Viability of cells after cold treatment was compared with viability of cells maintained at body temperature.
After 1 hour of cold treatment, SDFT core temperature was reduced by a mean of 21.8 degrees C, reaching a mean minimum temperature of 10 degrees C. Viability did not differ significantly between cold-treated and control cells.
Results indicated that topical application of cryotherapy significantly reduced core SDFT temperature in standing sedated horses. Temperatures achieved in vivo during cold treatment were not detrimental to the in vitro viability of tendon cells.
确定马匹在标准冷冻疗法应用过程中浅屈肌腱（SDFT）的冷却速率和程度，并评估冷却对肌腱细胞存活的体外影响。
5匹成年马的6条肢体以及在尸检时从3匹成年马的SDFT获取的培养细胞。
通过将热电偶温度探头插入每匹站立镇静马的前肢SDFT来获取体内数据。记录基线温度后，将带有循环冷却剂的商用加压夹板放置在每个选定的肢体上，然后对其进行60分钟的冷处理。每隔30秒记录一次温度。计算平均最低核心温度，并用于设计细胞体外冷处理方案。在尸检时从马的SDFT获取样本；将肌腱细胞悬浮培养，并暴露于模拟体内程序的1小时冷处理中。将冷处理后细胞的活力与维持在体温的细胞活力进行比较。
冷处理1小时后，SDFT核心温度平均降低21.8摄氏度，达到平均最低温度10摄氏度。冷处理细胞与对照细胞的活力无显著差异。
结果表明，在站立镇静的马匹中，局部应用冷冻疗法可显著降低SDFT核心温度。冷处理过程中在体内达到的温度对肌腱细胞的体外活力无害。
