Permingeat Hugo R, Alvarez María L, Cervigni Gerardo D L, Ravizzini Ricardo A, Vallejos Rubén H
Centro de Estudios Fotosintéticos y Bioquímicos (CONICET-Fund.M. Lillo-Universidad Nacional de Rosario), Suipacha 531, 2000 Rosario, Argentina.
Plant Mol Biol. 2003 May;52(2):415-9. doi: 10.1023/a:1023969501440.
Transgenic wheat plants without the selectable marker gene were obtained either in the presence or in the absence of selective pressure during the transformation protocol. When using hygromycin as selective agent in a co-transformation experiment involving a mixture of plasmids pGL2, containing the hpt gene, and pAI1Gus, containing the uidA gene, 3 out of 19 transgenic wheat plants had the uidA gene alone as shown by Southern blots. The gene was transmitted to the progeny following Mendelian rules. Segregation and loss of the selectable marker gene was also found in three out of six events from other experiments where high-molecular-weight glutenin genes were expressed or over-expressed. On the other hand, in 7 experiments where no selective pressure was applied and that involved 1016 bombarded explants, 23 transgenic wheat plants were obtained. The uidA gene was stably integrated as suggested by its transmission to the progeny.
在转化过程中，无论有无选择压力，均可获得不含选择标记基因的转基因小麦植株。在涉及含有潮霉素磷酸转移酶（hpt）基因的质粒pGL2和含有β-葡萄糖醛酸酶（uidA）基因的质粒pAI1Gus的共转化实验中，当使用潮霉素作为选择剂时，19株转基因小麦植株中有3株经Southern杂交显示仅含有uidA基因。该基因按孟德尔规律传递给后代。在其他表达或过表达高分子量谷蛋白基因的6个事件中，也有3个事件发现了选择标记基因的分离和丢失。另一方面，在7个未施加选择压力且涉及1016个轰击外植体的实验中，获得了23株转基因小麦植株。uidA基因向后代的传递表明其已稳定整合。
