Centro de Estudios Fotosintéticos y Bioquimicos, CEFOBI (CONICET, F.M. Lillo, Universidad Nacional de Rosario), Suipacha 531, 2000, Rosario, Argentina.
Plant Cell Rep. 1996 Sep;15(12):877-81. doi: 10.1007/BF00231579.
A highly efficient method for stable wheat transformation using hygromycin resistance as a selectable marker is described. Young embryogenic calli growing from immature wheat embryos were transformed using a gunpowder-driven microparticle accelerator. Transgenic wheat plants were determined by PCR amplification of transgene fragments and confirmed by Southern hybridization, activity of the transgene expression and by analysis of the progeny. The hpt gene was as good as or a better selectable marker than the bar gene with an average efficiency (number of transgenic plants relative to the number of bombarded calli) of 5.5% compared with 2.6% for the bar gene.
描述了一种利用潮霉素抗性作为选择标记进行稳定小麦转化的高效方法。从小麦幼胚中生长的幼胚性愈伤组织用火药驱动的微粒加速器进行转化。通过转基因片段的 PCR 扩增和Southern 杂交、转基因表达的活性以及对后代的分析来确定转基因小麦植株。与 bar 基因（平均效率为 2.6%）相比，hpt 基因作为选择标记的效果与 bar 基因一样好，甚至更好，相对于轰击的愈伤组织，其转基因植株的平均效率（转基因植株的数量与轰击的愈伤组织的数量之比）为 5.5%。
