Irie Sachiko, Saito Toyokazu, Kanazawa Naomi, Ogino Mieko, Ogino Yutaka, Sakai Fumihiko
Department of Neurology, Kitasato University School of Medicine, Sagamihara.
Intern Med. 2003 Jun;42(6):490-5. doi: 10.2169/internalmedicine.42.490.
Rapid detection of serum anti-ganglioside antibodies in Guillain-Barré syndrome (GBS) could facilitate early diagnosis and early initiation of treatment, which might shorten the term of illness and reduce sequelae. We examined serum anti-ganglioside antibodies in patients with GBS using the latex agglutination assay developed by Alaedini and Latov (J Immunoassay 21: 377-386, 2000) with some modifications.
We used 75 sera from GBS patients, which exhibited IgG anti-GM1, GD1b, or GQ1b, or IgM anti-GM2 antibodies on previous enzyme-linked immunosorbent assay (ELISA). Blue latex beads (2.5% solution of 0.3 microm) were coated with 1 mg/ml of GM1, GD1b, GQ1b or GM2. Aliquots (4 microl) of serum and the ganglioside-coated particles were mixed and rocked on a glass slide for 30 to 40 seconds. The reaction was observed under a microscope and compared with the antiganglioside antibody titers determined with ELISA.
Agglutination was strong in sera of which the IgM or IgG titers of anti-GM1, GD1b, GQ1b or GM2 antibodies were found to be more than 1:6,400 on ELISA except for 2 samples, but weak or absent in sera with titers of 1:3,200. Agglutination was absent in sera of which the antibody titers were less than 1:3,200 on ELISA.
We could rapidly detect serum IgM and IgG anti-GM1, GD1b, GQ1b and GM2 antibodies in patients with GBS by means of the latex agglutination assay when sera exhibited high titers of the respective antibodies on ELISA. The sensitivity of our agglutination assay was much lower than that of ELISA.
快速检测吉兰 - 巴雷综合征（GBS）患者血清中的抗神经节苷脂抗体有助于早期诊断和尽早开始治疗，这可能缩短病程并减少后遗症。我们采用经Alaedini和Latov改进的乳胶凝集试验（《免疫分析杂志》21: 377 - 386, 2000年）检测GBS患者血清中的抗神经节苷脂抗体。
我们使用了75份GBS患者的血清，这些血清在先前的酶联免疫吸附测定（ELISA）中显示出IgG抗GM1、GD1b或GQ1b，或IgM抗GM2抗体。将蓝色乳胶珠（0.3微米的2.5%溶液）用1毫克/毫升的GM1、GD1b、GQ1b或GM2包被。取4微升血清等分试样与包被神经节苷脂的颗粒混合，在载玻片上振荡30至40秒。在显微镜下观察反应，并与用ELISA测定的抗神经节苷脂抗体滴度进行比较。
除2份样本外，ELISA检测发现抗GM1、GD1b、GQ1b或GM2抗体的IgM或IgG滴度大于1:6400的血清中凝集反应强烈，但滴度为1:3200的血清中凝集反应较弱或无凝集反应。ELISA检测抗体滴度小于1:3200的血清中无凝集反应。
当血清在ELISA中显示出相应抗体的高滴度时，我们可以通过乳胶凝集试验快速检测GBS患者血清中的IgM和IgG抗GM1、GD1b、GQ1b和GM2抗体。我们的凝集试验灵敏度远低于ELISA。
