Umene Kenichi, Inoue Tomoyuki, Inoue Yoshitsugu, Shimomura Yoshikazu
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Med Virol. 2003 Sep;71(1):75-81. doi: 10.1002/jmv.10466.
Herpes simplex virus type 1 (HSV-1) is the etiological agent of herpetic keratitis. The epithelial form ("epithelial keratitis") is attributed mainly to destruction of the epithelium through active viral replication within the epithelium. The stromal form ("stromal keratitis") is associated with immune reactions within the stroma and is the common cause of human blindness. In the present study, 29 HSV-1 strains isolated from human ocular materials of herpetic keratitis were classified into 14 genotypes on the basis of DNA polymorphisms. Twenty-one of 29 (72%) strains from eyes examined in the present study were of genotypes that were shown previously to be present in strains from non-ocular lesions (including genital herpes). Five of nine (56%) strains from eyes related to stromal keratitis were of the F1 genotype, while four of twenty (20%) strains from eyes not related to stromal keratitis were of the F1 genotype. Thus, the proportion of F1 genotype was assumed to be larger in the group of strains related to stromal keratitis than in that not so related, suggesting an association of the F1 genotype with stromal keratitis. A connection of F1 genotype with recurrence was proposed previously; hence, F1 genotype seems to be associated to both stromal keratitis and the recurrence, thereby supporting the relationship between stromal keratitis and recurrence.
单纯疱疹病毒1型（HSV-1）是疱疹性角膜炎的病原体。上皮型（“上皮性角膜炎”）主要归因于上皮内活跃的病毒复制导致上皮破坏。基质型（“基质性角膜炎”）与基质内的免疫反应相关，是导致人类失明的常见原因。在本研究中，根据DNA多态性，从疱疹性角膜炎患者眼部材料中分离出的29株HSV-1毒株被分为14种基因型。本研究中检测的29株（72%）眼部分离株中有21株的基因型先前已在非眼部病变（包括生殖器疱疹）的毒株中出现。与基质性角膜炎相关的9株眼部分离株中有5株（56%）为F1基因型，而与基质性角膜炎无关的20株眼部分离株中有4株（20%）为F1基因型。因此，推测F1基因型在与基质性角膜炎相关的毒株组中的比例高于无关组，提示F1基因型与基质性角膜炎有关。先前有人提出F1基因型与复发有关；因此，F1基因型似乎与基质性角膜炎和复发均有关，从而支持了基质性角膜炎与复发之间的关系。
