Samelson R, Larkey D M, Amankwah K S, McConnachie P
Department of Obstetrics & Gynecology, Southern Illinois University School of Medicine, Springfield.
Am J Reprod Immunol. 1992 Sep;28(2):71-3. doi: 10.1111/j.1600-0897.1992.tb00761.x.
To determine the effect of labor on lymphocyte subsets in full-term neonates.
Cord blood obtained at delivery from full-term neonates, six born vaginally and six born by elective Cesarean section, was analyzed for lymphocyte subsets. Monoclonal antibodies, immunofluorescence, and flow cytometry were utilized to determine the lymphocyte phenotype frequencies in these neonates. These frequencies were compared by mode of delivery and to adult peripheral blood reference ranges using a two-tailed Student's t-test, P < 0.05.
A profile of significantly elevated T (CD2, CD3) and helper cells (CD4) and depressed Natural Killer cells (CD16, CD56) is characteristic of term Cesarean section neonates. Significantly depressed frequency of T cells (CD2, CD3) and helper T cells (CD4) and elevated Natural Killer cells (CD16, CD56) is characteristic of vaginally delivered neonates.
The mode of delivery affects the lymphocyte subset frequencies in full-term neonates.
确定分娩对足月儿淋巴细胞亚群的影响。
对足月新生儿分娩时采集的脐血进行淋巴细胞亚群分析，其中6例经阴道分娩，6例择期剖宫产。采用单克隆抗体、免疫荧光和流式细胞术测定这些新生儿的淋巴细胞表型频率。通过分娩方式比较这些频率，并使用双侧t检验与成人外周血参考范围进行比较，P < 0.05。
剖宫产足月儿的特征是T细胞（CD2、CD3）和辅助细胞（CD4）显著升高，自然杀伤细胞（CD16、CD56）降低。经阴道分娩新生儿的特征是T细胞（CD2、CD3）和辅助性T细胞（CD4）频率显著降低，自然杀伤细胞（CD16、CD56）升高。
分娩方式影响足月儿的淋巴细胞亚群频率。
