Kotiranta-Ainamo A, Apajasalo M, Pohjavuori M, Rautonen N, Rautonen J
Hospital for Children and Adolescents, University of Helsinki, Finland.
Clin Exp Immunol. 1999 Feb;115(2):309-14. doi: 10.1046/j.1365-2249.1999.00795.x.
Blood samples from 29 preterm (24-32 weeks of gestation) and 21 full-term (37-42 weeks of gestation) neonates were analysed for surface markers of lymphocyte subtypes and macrophages, and the effects of gestational age, neonatal infection, maternal pre-eclampsia, maternal betamethason therapy and mode of delivery were assessed with multiple regression analysis. Gestational age alone had few independent effects (increase in CD3+, CD8+CD45RA+, and CD11alpha+ cells, and decrease in CD14+, HLA-DR- cells) during the third trimester on the proportions of the immune cell subtypes studied. Neonatal infection and mother's pre-eclampsia had the broadest and very opposite kinds of effects on the profile of immune cells in the blood. Infection of the neonate increased the proportions of several 'immature' cells (CD11alpha-CD20+, CD40+CD19-, and CD14+HLA-DR-), whereas mother's pre-eclampsia decreased the proportions of naive cell types (CD4+CD8+, CD5+CD19+). In addition, neonatal infection increased the proportion of T cells (CD3+, CD3+CD25+, and CD4+/CD8+ ratio, and CD45RA+ cells), while maternal pre-eclampsia had a decreasing effect on the proportion of CD4+ cells, CD4+/CD8+ ratio, and proportions of CD11alpha+, CD14+ and CD14+HLA-DR+ cells. Maternal betamethason therapy increased the proportion of T cells (CD3+) and macrophages (CD14+, CD14+HLA-DR+), but decreased the proportion of natural killer (NK) cells. Caesarean section was associated with a decrease in the proportion of CD14+ cells. We conclude that the 'normal range' of proportions of different mononuclear cells is wide during the last trimester; further, the effect of gestational age on these proportions is more limited than the effects of other neonatal and even maternal factors.
对29例早产（妊娠24 - 32周）和21例足月（妊娠37 - 42周）新生儿的血样进行淋巴细胞亚群和巨噬细胞表面标志物分析，并通过多元回归分析评估胎龄、新生儿感染、母亲先兆子痫、母亲倍他米松治疗及分娩方式的影响。在妊娠晚期，仅胎龄对所研究的免疫细胞亚群比例的独立影响较小（CD3⁺、CD8⁺CD45RA⁺和CD11α⁺细胞增加，CD14⁺、HLA - DR⁻细胞减少）。新生儿感染和母亲先兆子痫对血液中免疫细胞谱的影响最为广泛且完全相反。新生儿感染增加了几种“未成熟”细胞的比例（CD11α⁻CD20⁺、CD40⁺CD19⁻和CD14⁺HLA - DR⁻），而母亲先兆子痫则降低了幼稚细胞类型的比例（CD4⁺CD8⁺、CD5⁺CD19⁺）。此外，新生儿感染增加了T细胞的比例（CD3⁺、CD3⁺CD25⁺以及CD4⁺/CD8⁺比值和CD45RA⁺细胞），而母亲先兆子痫对CD4⁺细胞比例、CD4⁺/CD8⁺比值以及CD11α⁺、CD14⁺和CD14⁺HLA - DR⁺细胞比例有降低作用。母亲倍他米松治疗增加了T细胞（CD3⁺）和巨噬细胞（CD14⁺、CD14⁺HLA - DR⁺）比例，但降低了自然杀伤（NK）细胞比例。剖宫产与CD14⁺细胞比例降低有关。我们得出结论，妊娠晚期不同单核细胞比例的“正常范围”较宽；此外，胎龄对这些比例的影响比其他新生儿甚至母亲因素的影响更有限。
