山东省四种菟丝子属植物的随机扩增多态性DNA分析
[RAPD analysis of four species of Cuscuta in Shandong Province].
作者信息
Lin Huibin, Lin Jianqun, Lin Jianqiang
机构信息
Shandong Institute of TCM, Jinan 250014.
出版信息
Zhong Yao Cai. 2003 Jan;26(1):8-10.
OBJECTIVE
To explore the genome difference of four species of Cuscuta in different hosts.
METHOD
RAPD was used by 50 primers.
RESULT
Four species of genus Cuscuta can be identified by 8 primers. Both Cuscuta chinensis and C. australis from Subg. Grammica had 3 bands whose molecular weights were 1.3 kb, 1.45 kb and 1.53 kb respectively. C. japonica and C. lupuliformis from Subg. Monogyna had a 1.48 kb specific band. Cuscuta of same subgenus had similar RAPD result and close genetic relationship. Same species of Cuscuta in different hosts showed DNA polymorphism. It indicated that hosts can affect genome of Cuscuta to some extent.
CONCLUSION
RAPD can be used to identify the species of Cuscuta or same Cuscuta in different hosts.
目的
探讨四种菟丝子在不同寄主上的基因组差异。
方法
使用50条引物进行随机扩增多态性DNA（RAPD）分析。
结果
8条引物可鉴别出四种菟丝子。隶属于线茎亚属的中国菟丝子和南方菟丝子均有3条带，分子量分别为1.3 kb、1.45 kb和1.53 kb。隶属于单柱亚属的日本菟丝子和啤酒花菟丝子有一条1.48 kb的特异性条带。同一亚属的菟丝子RAPD结果相似，亲缘关系较近。同一物种的菟丝子在不同寄主上表现出DNA多态性。这表明寄主在一定程度上会影响菟丝子的基因组。
结论
RAPD可用于鉴别菟丝子物种或同一物种在不同寄主上的差异。
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