用韩国株猪流行性腹泻病毒对仔猪进行实验性感染。

Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Kwanak-Gu 151-742, Seoul, South Korea.

J Comp Pathol. 2003 Jul;129(1):55-60. doi: 10.1016/s0021-9975(02)00170-6.

Thirty colostrum-deprived piglets aged 1 day were inoculated with a Korean strain of porcine epidemic diarrhoea virus (PEDV). The purpose was to elucidate the pathogenicity and viral distribution in PEDV-infected piglets over a period of 60 h, by morphometric analysis, in-situ hybridization and immunohistochemistry. At 24-60 h post-inoculation (hpi), the villous height/crypt depth (VH/CD) ratio of infected pigs was significantly less than that of control pigs. Positive cells typically exhibited a dark black reaction product (in-situ hybridization) or brown reaction product (immunohistochemistry) in the cytoplasm, without background staining. PEDV nucleic acid and antigen were detected in the duodenum, jejunum and ileum of experimentally infected pigs. The results suggested that the Korean strain was virulent and caused severe villous atrophy in the small intestine.

选取30头1日龄未食初乳的仔猪，接种一株韩国猪流行性腹泻病毒（PEDV）毒株。目的是通过形态计量分析、原位杂交和免疫组织化学方法，阐明PEDV感染仔猪60小时内的致病性和病毒分布情况。接种后24至60小时（hpi），感染猪的绒毛高度/隐窝深度（VH/CD）比值显著低于对照猪。阳性细胞的细胞质中通常呈现深黑色反应产物（原位杂交）或棕色反应产物（免疫组织化学），无背景染色。在实验感染猪的十二指肠、空肠和回肠中检测到PEDV核酸和抗原。结果表明，该韩国毒株具有致病性，可导致小肠严重绒毛萎缩。