Ellis J, Hassard L, Clark E, Harding J, Allan G, Willson P, Strokappe J, Martin K, McNeilly F, Meehan B, Todd D, Haines D
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon.
Can Vet J. 1998 Jan;39(1):44-51.
Postweaning multisystemic wasting syndrome (PMWS), an apparently new disease, has been recognized in swine herds in western Canada. Young pigs with this disease have progressive weight loss, tachypnea, dyspnea, and jaundice, accompanied by interstitial pneumonia, lymphadenopathy, hepatitis, and nephritis. We examined more than 400 pigs from more than 70 herds in Alberta, Saskatchewan, and Manitoba with cases of PMWS. A small virus was isolated from a range of tissues from 8 of 8 affected pigs examined. The agent was identified as a circovirus-like virus using electron microscopy, immunohistochemical staining with porcine and rabbit immune serum, and in situ hybridization. Immunohistochemical examination of tissues from more than 100 affected pigs has revealed widespread viral antigen, often contained in circovirus-like inclusion bodies, in lesions from numerous organs. Although Koch's postulates remain to be fulfilled, these results demonstrate a high degree of association between the presence of the circovirus-like virus and PMWS in affected swine.
断奶后多系统消耗综合征（PMWS）是一种明显的新疾病，已在加拿大西部的猪群中被发现。患这种病的仔猪会出现进行性体重减轻、呼吸急促、呼吸困难和黄疸，并伴有间质性肺炎、淋巴结病、肝炎和肾炎。我们检查了来自艾伯塔省、萨斯喀彻温省和曼尼托巴省70多个猪群的400多头患有PMWS的猪。从8头受检的患病猪的一系列组织中分离出一种小型病毒。利用电子显微镜、用猪和兔免疫血清进行免疫组织化学染色以及原位杂交，将该病原体鉴定为一种圆环病毒样病毒。对100多头患病猪的组织进行免疫组织化学检查发现，在许多器官的病变中存在广泛的病毒抗原，这些抗原通常包含在圆环病毒样包涵体内。尽管科赫法则仍有待验证，但这些结果表明，在受感染的猪中，圆环病毒样病毒的存在与PMWS之间存在高度关联。
