Liu Xinsheng, Lin Chun-Ming, Annamalai Thavamathi, Gao Xiang, Lu Zhongyan, Esseili Malak A, Jung Kwonil, El-Tholoth Mohamed, Saif Linda J, Wang Qiuhong
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA.
State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Vet Res. 2015 Sep 25;46:109. doi: 10.1186/s13567-015-0249-1.
The infectious dose of a virus pool of original US PEDV strain PC22A was determined in 4-day-old, cesarean-derived, colostrum-deprived (CDCD) piglets. The median pig diarrhea dose (PDD50) of the virus pool was determined as 7.35 log10 PDD50/mL, similar to the cell culture infectious titer, 7.75 log10 plaque-forming units (PFU)/mL. 100 PDD50 caused watery diarrhea in all conventional suckling piglets (n = 12) derived from a PEDV-naive sow, whereas 1000 and 10 000 PDD50 did not cause diarrhea in piglets derived from two PEDV-field exposed-recovered sows. This information is important for future PEDV challenge studies and validation of PEDV vaccines.
在美国原猪流行性腹泻病毒（PEDV）毒株PC22A的病毒库的感染剂量在4日龄剖腹产、未摄入初乳（CDCD）的仔猪中进行了测定。该病毒库的猪腹泻剂量中位数（PDD50）被确定为7.35 log10 PDD50/mL，与细胞培养感染滴度7.75 log10空斑形成单位（PFU）/mL相似。100 PDD50使来自未接触过PEDV的母猪的所有常规哺乳仔猪（n = 12）出现水样腹泻，而1000和10000 PDD50并未使来自两头曾接触过PEDV并康复的母猪的仔猪出现腹泻。这些信息对于未来的PEDV攻毒研究和PEDV疫苗的验证很重要。
