Wang G, Song G, Becker W
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Chin Med Sci J. 1992 Sep;7(3):148-51.
To elucidate the importance of glucose metabolism in Oncomelania snails, uniformly labelled D (14C)-glucose [(U-14C)-glucose] has been used as a tracer for observing glucose uptake, incorporation into macromolecules and the activity of enzymes involved in its metabolic pathway. Radioactivity of Oncomelania increased with time of exposure (3, 6, 12 and 24 h) to [(U-14C)-glucose] solution. In snails treated with 2 ppm bromoacetamide for 24 h, the radioactivity of soft tissues was markedly less than that of normal snails. Oncomelania can synthesize glycogen from absorbed glucose, and the absorbed (U-14C)-glucose may be incorporated into nucleic acid, protein and lipid, with 14CO2 excreted as an end metabolic product. 6-phosphoglucose dehydrogenase and 6-phosphogluconate dehydrogenase activities were demonstrated by the reduction of NADP and determination of 14CO2 from (1-14C)-glucose, respectively. The results indicate that there exists a phosphopentose pathway in Oncomelania.
为了阐明葡萄糖代谢在钉螺中的重要性，均匀标记的D-（14C）-葡萄糖[（U-14C）-葡萄糖]已被用作示踪剂，用于观察葡萄糖摄取、掺入大分子以及参与其代谢途径的酶的活性。钉螺的放射性随着暴露于[（U-14C）-葡萄糖]溶液的时间（3、6、12和24小时）而增加。在用2 ppm溴乙酰胺处理24小时的钉螺中，软组织的放射性明显低于正常钉螺。钉螺可以从吸收的葡萄糖合成糖原，吸收的（U-14C）-葡萄糖可能掺入核酸、蛋白质和脂质中，14CO2作为最终代谢产物排出。分别通过NADP的还原和从（1-14C）-葡萄糖测定14CO2来证明6-磷酸葡萄糖脱氢酶和6-磷酸葡萄糖酸脱氢酶的活性。结果表明钉螺中存在磷酸戊糖途径。
