Moro L, Martins A S, Alves C M, Santos F G A, Del Puerto H L, Vasconcelos A C
Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31 270-901, Belo Horizonte, Brasil.
J Vet Med B Infect Dis Vet Public Health. 2003 Jun;50(5):221-5. doi: 10.1046/j.1439-0450.2003.00657.x.
Canine distemper virus (CDV) may induce multifocal demyelination in the central nervous system of infected dogs. The pathogenesis of this process is not clear. The present work identifies the presence of apoptotic cells in white and grey matter of dogs'cerebellum, naturally infected with CDV. Fifteen dogs with clinical signs of canine distemper that tested positive for CDV nucleoprotein were used. Brain specimens were processed and embedded in paraffin. Sections 5 microm thick were stained with hematoxylin-eosin and Shorr. Other sections were submitted to TUNEL reaction and to immunohistochemistry for CDV nucleoprotein detection. Acute and chronic demyelinated plaques were observed in the white matter, while apoptosis occurred particularly in the granular layer of grey matter. Apoptosis seems to play an important role in the pathogenesis of canine distemper demyelination.
犬瘟热病毒（CDV）可在感染犬的中枢神经系统中引发多灶性脱髓鞘。这一过程的发病机制尚不清楚。目前的研究确定了自然感染CDV的犬小脑白质和灰质中存在凋亡细胞。使用了15只出现犬瘟热临床症状且CDV核蛋白检测呈阳性的犬。对脑标本进行处理并嵌入石蜡。5微米厚的切片用苏木精-伊红和肖尔染色。其他切片进行TUNEL反应和免疫组织化学检测以检测CDV核蛋白。在白质中观察到急性和慢性脱髓鞘斑块，而凋亡尤其发生在灰质的颗粒层。凋亡似乎在犬瘟热脱髓鞘的发病机制中起重要作用。
