Jang Mi-Hyeon, Shin Min-Chul, Lee Taeck-Hyun, Lim Baek-Vin, Shin Mal-Soon, Min Byung-Il, Kim Hong, Cho Sonhae, Kim Ee-Hwa, Kim Chang-Ju
Department of Physiology, College of Medicine, Kyung Hee University, # 1 Hoigi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
Neurosci Lett. 2003 Aug 14;347(1):5-8. doi: 10.1016/s0304-3940(03)00512-3.
Acupuncture has been used for the enhancement of functional recovery from various disorders including stroke. In the present study, the effects of acupuncture on the c-Fos expression and apoptosis in the hippocampal CA1 region of gerbils following transient global ischemia were investigated via immunohistochemistry for c-Fos and caspase-3 and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Enhanced Fos, TUNEL, and caspase-3 positivities were detected in the hippocampal CA1 region in the ischemic gerbils. Acupunctural treatment suppressed the ischemia-induced increment in the number of Fos-, TUNEL-, and caspase-3-positive cells: the most potent suppressive effect was observed at the Zusanli acupoint. These results suggest that acupunctural treatment alleviates ischemia-induced apoptosis and may aid in the recovery following ischemic cerebral injury.
针刺已被用于促进包括中风在内的各种疾病后的功能恢复。在本研究中，通过对c-Fos和半胱天冬酶-3进行免疫组织化学以及末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）分析，研究了针刺对沙土鼠短暂性全脑缺血后海马CA1区c-Fos表达和细胞凋亡的影响。在缺血沙土鼠的海马CA1区检测到Fos、TUNEL和半胱天冬酶-3阳性增强。针刺治疗抑制了缺血诱导的Fos、TUNEL和半胱天冬酶-3阳性细胞数量的增加：在足三里穴位观察到最有效的抑制作用。这些结果表明，针刺治疗可减轻缺血诱导的细胞凋亡，并可能有助于缺血性脑损伤后的恢复。
