Department of Pediatrics, The Third Affiliated Hospital of Zhengzhou University, Kangfuqain Street 7, Zhengzhou 450052, China.
Neurol Sci. 2011 Jun;32(3):369-74. doi: 10.1007/s10072-010-0402-6. Epub 2010 Sep 18.
To investigate the effect of electroacupuncture on cell proliferation and differentiation in young rat hippocampus, postnatal day-14 rats were assigned randomly to control, ketamine anesthesia, nonacupoint, or acupoint electroacupuncture groups. Electroacupuncture was applied at bilateral acupoints (Quchi, Waiguan, Huantiao, and Zusanli) 30 min daily for 7 successive days. The proliferation and neuronal differentiation of proliferated surviving cells in the dentate gyrus were evaluated at 4 weeks after last stimulation. The stimulatory effect of electroacupuncture on cell proliferation had a long-lasting effect, as indicated by the increased phosphor-histone H3-positive cells. The number of proliferated and survival cells, indicated by BrdU labeling, was highest in the electroacupuncture group. The number of newly differentiated neurons, as indicated by BrdU/NeuN double labeling, was significantly higher in the electroacupuncture group than in any of the other groups. This finding provides a theoretical basis for the clinical application of acupuncture to cerebral injury rehabilitation in children.
为了研究电针对幼年大鼠海马细胞增殖和分化的影响，将出生后 14 天的大鼠随机分为对照组、氯胺酮麻醉组、非穴位组和穴位电针组。电针每日双侧穴位（曲池、外关、环跳和足三里）刺激 30 分钟，连续 7 天。末次刺激后 4 周评估齿状回内增殖存活细胞的增殖和神经元分化情况。电针对细胞增殖的刺激作用具有持久的效果，表现为磷酸组蛋白 H3 阳性细胞增多。BrdU 标记的增殖和存活细胞数量在电针组中最高。BrdU/NeuN 双标记的新分化神经元数量在电针组中明显高于其他各组。这一发现为临床应用针刺治疗儿童脑损伤康复提供了理论依据。
