Jiang Chunhui, Fogel Ronald, Zhang Xueguo
Laboratory of Neurogastroenterology Research, Division of Gastroenterology, Henry Ford Health System, One Ford Place 2D, 6071 Second Avenue, Detroit, MI 48202, USA.
Brain Res. 2003 Aug 1;980(1):31-47. doi: 10.1016/s0006-8993(03)02844-0.
The lateral hypothalamus (LH) regulates metabolic, behavioral and autonomic functions. The influence of the LH on gastrointestinal function and feeding behavior may be mediated by the dorsal vagal complex (DVC). In the present experiment, we used tract tracing and neurophysiologic techniques to evaluate the interrelationship between the LH and DVC. Using the tracer DiI, we demonstrated that the LH projects to both the nucleus of the solitary tract (NST) and the dorsal motor nucleus of the vagus (DMNV). We determined the effects of electrical stimulation of the LH and/or distention of the gastrointestinal tract on the firing rates of 107 DMNV neurons and 68 NST neurons. As previously reported, the majority of the DMNV neurons were inhibited and the majority of the NST neurons were excited by gastrointestinal distention. Electrical stimulation of the LH significantly changed the spontaneous activities of 71% of the DMNV neurons (46 excited and 30 inhibited). Of the 68 NST neurons characterized, 25 neurons were inhibited and 8 were excited by LH stimulation. In a separate experiment, we characterized the effects of both electrical and chemical stimulation of the LH on 36 DMNV and 14 NST neurons. Glutamate (0.8 nM) induced similar responses in the DVC neurons as electrical stimulation of the LH. The results indicate that the LH influences the electrical activity of DVC neurons. This effect may be the mechanism by which the LH modulates gastrointestinal function and feeding behavior.
外侧下丘脑（LH）调节代谢、行为和自主功能。LH对胃肠功能和摄食行为的影响可能由迷走神经背侧复合体（DVC）介导。在本实验中，我们使用示踪追踪和神经生理学技术来评估LH和DVC之间的相互关系。使用示踪剂DiI，我们证明LH投射到孤束核（NST）和迷走神经背运动核（DMNV）。我们确定了电刺激LH和/或胃肠道扩张对107个DMNV神经元和68个NST神经元放电率的影响。如先前报道，胃肠道扩张抑制了大多数DMNV神经元，兴奋了大多数NST神经元。电刺激LH显著改变了71%的DMNV神经元的自发活动（46个兴奋，30个抑制）。在68个已鉴定的NST神经元中，25个神经元被LH刺激抑制，8个被兴奋。在另一个实验中我们确定了LH的电刺激和化学刺激对36个DMNV神经元和14个NST神经元的影响。谷氨酸（0.8 nM）在DVC神经元中引起的反应与LH的电刺激相似。结果表明，LH影响DVC神经元的电活动。这种作用可能是LH调节胃肠功能和摄食行为的机制。
