Zhang Qian, Cai Lifen, Zhong Guoyue, Luo Weizao
National Engineering Laboratory for Propagation of Endangered Traditional Chinese Medicine, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2061-4.
To develop a HPLC method to determine the content of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex simultaneously. MEHTOD: The separations were carried out at 25 degrees C on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitril and water containing 0.1% phosphoric acid in gradient mode. The flow rate was 1.0 mL x min(-1), detection wavelengthes were 345 nm for jatrorrhizine , palmatine, berberine and 210 nm for obacunone.
The average recoveries of jatrorrhizine, palmatine, berberine, and obacunone were 98.94%, 101.17%, 96.22% and 98.90%, respectively.
The method is simple, accurate and repeatable, and can be used in content determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex.
建立同时测定黄柏中盐酸小檗碱、巴马汀、盐酸巴马汀和奥巴库酮含量的高效液相色谱法。方法：在25℃下，采用菲罗门Gemini C18柱（4.6mm×250mm，5μm），以乙腈和含0.1%磷酸的水为流动相进行梯度洗脱。流速为1.0mL·min-1，盐酸小檗碱、巴马汀、盐酸巴马汀的检测波长为345nm，奥巴库酮的检测波长为210nm。结果：盐酸小檗碱、巴马汀、盐酸巴马汀和奥巴库酮的平均回收率分别为98.94%、101.17%、96.22%和98.90%。结论：该方法简便、准确、重复性好，可用于黄柏中盐酸小檗碱、巴马汀、盐酸巴马汀和奥巴库酮的含量测定。
