Cui X M, Lo C K, Yip K L, Dong T T, Tsim K W
Department of Biology and Biotechnology Research Institute, The Hong Kong University of Science and Technology, Hong Kong, P. R. China.
Planta Med. 2003 Jun;69(6):584-6. doi: 10.1055/s-2003-40632.
The great majority of Panax species are well-known herbal medicines in the Orient, and many of them share a close resemblance in appearance and chemical composition. Among these Panax species, the root of P. notoginseng (Sanqi) is a unique herb that has distinct clinical usage. Here, the 5S-rRNA spacer domains were isolated from P. notoginseng, P. japonicus var. major, P. stipuleanatus, P. quinquefolius, P. ginseng, P. zingiberensis, and P. wangianus, and four common adulterants of P. notoginseng including Curcuma wenyujin, Curcuma longa, Bletilla striata and Gynura segetum. The spacer domains were sequenced and compared, which showed over 75 % DNA identity among all Panax species, but not for the adulterants. In addition, random amplification of polymorphic DNA (RAPD) analysis was used to distinguish different members of Panax genus as well as the morphological variants of P. notoginseng. These molecular methods could be used in the authentic identification of P. notoginseng from other Panax species.
大多数人参属植物是东方著名的草药，其中许多在外观和化学成分上极为相似。在这些人参属植物中，三七的根是一种具有独特临床用途的独特草药。在此，从三七、大叶三七、屏边三七、西洋参、人参、姜状三七和越南人参以及三七的四种常见伪品温郁金、姜黄、白及和菊三七中分离出5S - rRNA间隔区。对间隔区进行测序和比较，结果显示所有人参属植物之间的DNA同源性超过75%，但伪品则不然。此外，利用随机扩增多态性DNA（RAPD）分析来区分人参属的不同成员以及三七的形态变异体。这些分子方法可用于三七与其他人参属植物的正品鉴定。
