应用序列特征性扩增区域（SCAR）分析鉴定人参属植物及其掺伪品。
Application of sequence characterized amplified region (SCAR) analysis to authenticate Panax species and their adulterants.
作者信息
Wang J, Ha W Y, Ngan F N, But P P, Shaw P C
机构信息
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
出版信息
Planta Med. 2001 Nov;67(8):781-3. doi: 10.1055/s-2001-18340.
PMID:11731932
Abstract
A 420-bp RAPD fragment from Panax quinquefolius was converted to a sequence characterized amplified region (SCAR) marker. The main difference between the SCAR of P. quinquefolius and its homolog in P. ginseng is the presence of a 25 bp insertion in the latter. Primers derived from this sequence were successfully used to authenticate six Panax species and two common adulterants.
摘要
西洋参的一个420 bp随机扩增多态性DNA（RAPD）片段被转化为序列特异性扩增区域（SCAR）标记。西洋参的SCAR与其人参同源物之间的主要差异在于后者存在一个25 bp的插入序列。从该序列衍生的引物成功用于六种人参属植物和两种常见掺假品的鉴定。
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