Shaw P C, But P P
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N. T.
Planta Med. 1995 Oct;61(5):466-9. doi: 10.1055/s-2006-958138.
DNA from dried or fresh roots of three medicinal Panax species and their adulterants were amplified by AP-PCR or RAPD. Fingerprints for P. ginseng or P. quinquefolius were found to be consistent irrespective of plant source or age. On the other hand, very different fingerprints were obtained from adulterants. Hence the PCR approach may be used as a means to authenticate the concerned Panax species. Analysis of the degree of similarity of the fingerprints confirmed that P. ginseng is more closely related to P. quinquefolius than to P. notoginseng.
通过AP-PCR或RAPD对三种药用人参属植物及其掺假品的干根或鲜根DNA进行扩增。结果发现,人参或西洋参的指纹图谱无论植物来源或年龄如何都是一致的。另一方面,从掺假品中获得的指纹图谱却大不相同。因此,PCR方法可作为鉴定相关人参属植物的一种手段。指纹图谱相似度分析证实,人参与西洋参的亲缘关系比与人参三七更近。