Tripathi A, Dasgupta S, Roy A, Sengupta A, Roy B, Roychowdhury S, Panda C K
Dept. of Oncogene Regulation, Chittaranjan National Cancer Institute, Calcutta, India.
J Exp Clin Cancer Res. 2003 Jun;22(2):289-97.
In the deletion mapping of chromosome (chr) 9 in head and neck lesions of the Indian patient population by microsatellite markers, we have identified four discrete areas (D1-D4) with high loss of heterozygosities (LOHs) viz. 9p24-p23 (D1), 9p22-p21 (D2), 9q11-q13 (D3) and 9q22.3 (D4) regions. The deletions in D2 and D4 regions were suggested to be essential for the development of dysplastic lesions of head and neck, whereas the deletions in D1 and D3 regions were responsible for progression of the dysplastic lesions to early invasive head and neck squamous cell carcinoma (HNSCC). The microsatellite size alterations (MAs) were observed in the chromosomal 9pter-p23, 9p22-p21(D2), 9q13 and 9q21.1-q21.2 regions with gradual increase during progression of the tumor. Additional chromosomal alterations like loss of normal copy of chr.9 and biallelic alterations were also seen in our samples. There is a correlation between HPV infection with TNM stages, histopathological grades and LOHs at D1 and D4 regions. Whereas tobacco habit is associated with the occurrence of LOHs at D1 and LOHs / MAs at D2 region.
在通过微卫星标记对印度患者群体头颈部病变中的9号染色体（chr）进行缺失图谱分析时，我们确定了四个杂合性高缺失（LOH）的离散区域（D1 - D4），即9p24 - p23（D1）、9p22 - p21（D2）、9q11 - q13（D3）和9q22.3（D4）区域。D2和D4区域的缺失被认为对头颈部发育异常病变的发展至关重要，而D1和D3区域的缺失则导致发育异常病变进展为早期浸润性头颈部鳞状细胞癌（HNSCC）。在染色体9pter - p23、9p22 - p21（D2）、9q13和9q21.1 - q21.2区域观察到微卫星大小改变（MA），且在肿瘤进展过程中逐渐增加。在我们的样本中还观察到其他染色体改变，如9号染色体正常拷贝的缺失和双等位基因改变。HPV感染与TNM分期、组织病理学分级以及D1和D4区域的LOH之间存在相关性。而吸烟习惯与D1区域的LOH以及D2区域的LOH / MA的发生有关。
