Ah-See K W, Cooke T G, Pickford I R, Soutar D, Balmain A
Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow.
Cancer Res. 1994 Apr 1;54(7):1617-21.
The detection in tumors of genomic regions with a high frequency of loss of heterozygosity has led to the localization and subsequent cloning of a number of tumour suppressor genes. To identify such regions involved in the development of squamous carcinoma of the head and neck we have analyzed 28 paired normal and tumor DNA samples. Using the polymerase chain reaction to amplify 50 simple sequence repeats or microsatellite markers we have studied all 22 q limbs and 17 of the p limbs in 21 patients. In informative cases we observed a high incidence of loss of heterozygosity at five specific chromosomal regions: 3p (44%); 5q (43%); 9q (35%); 11q (45%); and 17p (31%). In addition, further analysis of tumors showing loss of heterozygosity at 5q suggests that a gene at or near the APC locus is involved in squamous carcinoma of the head and neck.
在肿瘤中检测到杂合性缺失频率较高的基因组区域，已促使人们定位并随后克隆了许多肿瘤抑制基因。为了鉴定参与头颈部鳞状细胞癌发生发展的此类区域，我们分析了28对正常和肿瘤DNA样本。利用聚合酶链反应扩增50个简单序列重复或微卫星标记，我们研究了21例患者的所有22号染色体q臂以及17例患者的17号染色体p臂。在信息充分的病例中，我们在五个特定染色体区域观察到较高的杂合性缺失发生率：3p（44%）；5q（43%）；9q（35%）；11q（45%）；以及17p（31%）。此外，对在5q处显示杂合性缺失的肿瘤进行的进一步分析表明，APC基因座或其附近的一个基因参与了头颈部鳞状细胞癌的发生。
