VR1与VRL-1在大鼠迷走神经感觉神经节中的共表达。

The co-expression of VR1 and VRL-1 in the rat vagal sensory ganglia.

作者信息

Ichikawa H, Sugimoto T

机构信息

Department of Oral Function and Anatomy, and Biodental Research Center, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8525, Japan.

出版信息

Brain Res. 2003 Aug 8;980(2):293-6. doi: 10.1016/s0006-8993(03)02998-6.

DOI:10.1016/s0006-8993(03)02998-6

PMID:12867271

Abstract

Immunohistochemistry for two nociceptive transducers, the vanilloid receptor 1 (VR1) and vanilloid receptor 1-like receptor (VRL-1), was performed on the vagal sensory ganglia. In the jugular ganglion, VR1-immunoreactive (IR) neurons were small to medium-sized (range 49.7-1,125.6 microm(2), mean+/-S.D. 407.7+/-219.7 microm(2)), whereas VRL-1-IR neurons were medium-sized to large (range 223.6-1,341.1 microm(2), mean+/-S.D. 584.3+/-253.5 microm(2)). In the nodose ganglion, VR1- and VRL-1-IR neurons were mostly small to medium-sized (VR1: range 148.5-1464.4 microm(2), mean+/-S.D. 554.3+/-207.4 microm(2); VRL-1: range 161.7-1166.2 microm(2), mean+/-S.D. 541.9+/-186.2 microm(2)). The double immunofluorescence method revealed that co-expression of VR1-immunoreactivity among VRL-1-IR neurons was more abundant in the nodose ganglion (63%) than in the jugular ganglion (4%). The present study suggests that co-expression of VR1 and VRL-1 may be more common in visceral sensory neurons than in somatic sensory neurons.

摘要

对两个伤害性感受器，即香草酸受体1（VR1）和类香草酸受体1样受体（VRL-1）进行免疫组织化学检测，检测部位为迷走神经感觉神经节。在颈神经节中，VR1免疫反应性（IR）神经元为中小型（范围49.7 - 1,125.6平方微米，平均值±标准差407.7±219.7平方微米），而VRL-1-IR神经元为中型到大型（范围223.6 - 1,341.1平方微米，平均值±标准差584.3±253.5平方微米）。在结状神经节中，VR1-IR和VRL-1-IR神经元大多为中小型（VR1：范围148.5 - 1464.4平方微米，平均值±标准差554.3±207.4平方微米；VRL-1：范围161.7 - 1166.2平方微米，平均值±标准差541.9±186.2平方微米）。双重免疫荧光法显示，VRL-1-IR神经元中VR1免疫反应性的共表达在结状神经节（63%）中比在颈神经节（4%）中更丰富。本研究表明，VR1和VRL-1的共表达在内脏感觉神经元中可能比在躯体感觉神经元中更常见。