Oh Kwi Ok, Kim Se Won, Kim Jong Yeo, Ko Seon Yle, Kim Hyun Man, Baek Jeong Hwa, Ryoo Hyun Mo, Kim Jung Keun
OCT Inc., 2-17, Omok-ri, Seonggeo-eup, Choongnam 330-831, Chonan, South Korea.
Clin Chim Acta. 2003 Aug;334(1-2):185-95. doi: 10.1016/s0009-8981(03)00238-9.
Rehmannia glutinosa Libosch extracts (RGX) were investigated to determine if they play roles in bone metabolism.
The effects on osteoblasts were determined by measuring (1) cell proliferation, (2) alkaline phosphatase (ALP) activity, (3) mRNA expression of bone-related proteins, (4) transcriptional activity of Runx2, and (5) osteoprotegerin (OPG) secretion. The effects on the osteoclasts were investigated by measuring (1) tartrate-resistant acid phosphatase-positive [TRAP(+)] multinucleated cell (MNC) formation and (2) resorption areas after culturing osteoclast precursors. Bone mineral density (BMD) measurements and histological observations on rats were also carried out.
RGX treatment showed a significant increase in both the proliferation and ALP activity of osteoblasts. RGX increased the expression of the bone-related genes. OPG secretion was markedly increased after RGX treatment. In addition, RGX treatment decreased the number of TRAP(+) MNCs and the resorption areas. In vivo studies using ovariectomy-induced osteoporotic rats revealed that RGX alleviated the decrease in the trabecular BMD, and increased the cortical bone thickness and trabeculation of the bone marrow spaces.
RGX stimulates the proliferation and activities of osteoblasts, while inhibiting the generation and resorptive activities of osteoclasts. It also shows preventive effects on osteoporotic bone loss induced by an ovariectomy. Although the active substances have not yet been identified, it is believed that the RGX seems to contain active components that have a potential to enhance the bone metabolism in osteoporosis.
对地黄提取物（RGX）进行研究，以确定其是否在骨代谢中发挥作用。
通过测量以下指标来确定对成骨细胞的影响：（1）细胞增殖；（2）碱性磷酸酶（ALP）活性；（3）骨相关蛋白的mRNA表达；（4）Runx2的转录活性；（5）骨保护素（OPG）分泌。通过测量以下指标来研究对破骨细胞的影响：（1）抗酒石酸酸性磷酸酶阳性[TRAP(+)]多核细胞（MNC）形成；（2）培养破骨细胞前体后的吸收面积。还对大鼠进行了骨密度（BMD）测量和组织学观察。
RGX处理使成骨细胞的增殖和ALP活性均显著增加。RGX增加了骨相关基因的表达。RGX处理后OPG分泌明显增加。此外，RGX处理减少了TRAP(+) MNC的数量和吸收面积。使用去卵巢诱导的骨质疏松大鼠进行的体内研究表明，RGX减轻了小梁骨密度的降低，并增加了皮质骨厚度和骨髓腔的小梁化。
RGX刺激成骨细胞的增殖和活性，同时抑制破骨细胞的生成和吸收活性。它还对去卵巢诱导的骨质疏松性骨丢失具有预防作用。尽管尚未确定活性物质，但据信RGX似乎含有具有增强骨质疏松症中骨代谢潜力的活性成分。
