Balasundaram D, Dinman J D, Wickner R B, Tabor C W, Tabor H
Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):172-6. doi: 10.1073/pnas.91.1.172.
Polyamines have been implicated in nucleic acid-related functions and in protein biosynthesis. RNA sequences that specifically direct ribosomes to shift reading frame in the -1 and +1 directions may be used to probe the mechanisms controlling translational fidelity. We examined the effects of spermidine on translational fidelity by an in vivo assay in which changes in beta-galactosidase activity are dependent on yeast retrovirus Ty +1 and yeast double-stranded RNA virus L-A -1 ribosomal frameshifting signals. In spe2 delta mutants of Saccharomyces cerevisiae, which cannot make spermidine as a result of a deletion in the SPE2 gene, there is a marked elevation in +1 but no change in -1 ribosomal frameshifting. The increase in +1 ribosomal frameshifting efficiency is accompanied by a striking decrease in Ty1 retrotransposition.
多胺与核酸相关功能及蛋白质生物合成有关。能够特异性引导核糖体在-1和+1方向上移动阅读框的RNA序列可用于探究控制翻译保真度的机制。我们通过体内实验检测了亚精胺对翻译保真度的影响，在该实验中，β-半乳糖苷酶活性的变化取决于酵母逆转录病毒Ty +1和酵母双链RNA病毒L-A -1核糖体移码信号。在酿酒酵母的spe2Δ突变体中，由于SPE2基因缺失而无法合成亚精胺，+1核糖体移码显著升高，但-1核糖体移码没有变化。+1核糖体移码效率的增加伴随着Ty1逆转座的显著降低。
