Xu S, Wang J, Wen L, Shi C X, Zhang Z H, Xia S Y, He Y Q, Li N, Yue H J, Hong T
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2001 Sep;15(3):269-73.
To study the genetic variations of the major neutralization antigen VP7 of group A rotavirus, type G1 in China.
Twenty-three isolates derived from seven cities (Beijing, Shenyang, Xinxiang, Shanghai, Shenzhen, Guangzhou and Chongqing) during the period of 1988-1998 were analyzed for the VP7 cDNA sequences.
An obvious homology was observed in amino acid sequences of VP7 in the 23 isolates. Compared with the standard strain Wa, the variations were found at the position of aa 41, 49, 57, 65, 68, 74, 94, 97, 147, 170, 217, 218, 268, 281 and 291, respectively, and all of these were located in the variable region(VR)3,4,5,7,8 as well as in the C-terminal of the peptide. There seemed to be no remarkable divergence among the samples collected from different cities and all of them shared the same genetic lineage with the strain Jpn-417, though there might be one or two amino acid substitutions as time passed. When detected simultaneously with ELISA using G1 serotype-specific monoclonal antibody, the location of amino acid 91 was found presumably related to the neutralization epitope in VP7 of the type G1. Variations at some position may be resulted in the change of electropherotype.
The results indicated that the recent isolates with representative genetic and antigenic features should be used to develop effective vaccines, and the antigenic variations should be monitored periodically.
研究中国G1型A组轮状病毒主要中和抗原VP7的基因变异情况。
对1988 - 1998年期间从7个城市（北京、沈阳、新乡、上海、深圳、广州和重庆）分离出的23株病毒进行VP7 cDNA序列分析。
23株病毒VP7的氨基酸序列具有明显的同源性。与标准株Wa相比，分别在第41、49、57、65、68、74、94、97、147、170、217、218、268、281和291位氨基酸处发现变异，这些变异均位于可变区（VR）3、4、5、7、8以及肽段的C末端。不同城市分离的毒株之间似乎没有明显差异，它们与Jpn - 417株具有相同的遗传谱系，不过随着时间推移可能会有一两个氨基酸替换。用G1血清型特异性单克隆抗体进行ELISA检测时，发现91位氨基酸的位置可能与G1型VP7的中和表位有关。某些位置的变异可能导致电泳图谱型的改变。
结果表明，应使用具有代表性遗传和抗原特征的近期分离株来研发有效的疫苗，并定期监测抗原变异情况。
