Kislinger Thomas, Emili Andrew
Program in Proteomics and Bioinformatics, Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, ON M5G 1L6, Canada.
Curr Opin Mol Ther. 2003 Jun;5(3):285-93.
Protein expression profiling, the science of monitoring global sets of proteins produced by any given cell type, tissue or organism, has been invigorated by the introduction of proteomic technologies capable of characterizing large numbers of proteins. This review summarizes recent advances in mass spectrometry-based techniques for high-throughput protein identification and quantitation that are fueling rapid growth in the field. Key publications applying state-of-the-art 'shotgun' methods for investigating the entire protein complement of whole organelles, cells and tissues are highlighted. An overview of current proteomic challenges, particularly in the area of data analysis, and the long-term prospects of protein profiling strategies in basic biomedical research, therapeutics development and clinical discovery is also provided.
蛋白质表达谱分析，即监测任何特定细胞类型、组织或生物体所产生的全部蛋白质组的科学，因能够鉴定大量蛋白质的蛋白质组学技术的引入而得到振兴。本综述总结了基于质谱技术在高通量蛋白质鉴定和定量方面的最新进展，这些进展推动了该领域的快速发展。重点介绍了应用最先进的“鸟枪法”方法研究整个细胞器、细胞和组织的完整蛋白质组的关键出版物。还概述了当前蛋白质组学面临的挑战，特别是在数据分析领域，以及蛋白质谱分析策略在基础生物医学研究、治疗药物开发和临床发现方面的长期前景。
