Imamura Shigeyoshi, Kita Masakazu, Yamaoka Yoshio, Yamamoto Toshiro, Ishimaru Atsushi, Konishi Hideyuki, Wakabayashi Naoki, Mitsufuji Shoji, Okanoue Takeshi, Imanishi Jiro
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Am J Gastroenterol. 2003 Jul;98(7):1500-3. doi: 10.1111/j.1572-0241.2003.07516.x.
The routes of human infection with Helicobacter pylori remain unclear. In the present study, we examined cockroaches as possible vectors for transmission of H. pylori.
We used a common species of cockroach (Periplaneta fuliginosa). After a 3-day fast, cockroaches were placed on agar plates containing freshly grown H. pylori (Sydney strain) (challenge group) or on sterile agar plates without H. pylori (control group). After 24 h of challenge, cockroaches were moved to disinfected containers, and sterile food and water were provided. The external surfaces (legs and body) and excreta of the cockroaches were sampled for culture, rapid urease test, and polymerase chain reaction (PCR).
H. pylori were culturable from the excreta of the challenge group for 24 h postchallenge. Positive rapid urease test results were obtained up to day 3, and PCR analysis was positive for H. pylori DNA up to day 7 from the excreta. In contrast, H. pylori were not culturable from the external surfaces of the cockroaches. The rapid urease test was positive for only 8 h, and PCR analysis was positive for H. pylori DNA for 1 day from the external surface.
Cockroaches usually live in unsanitary environments and may contaminate foods and food containment areas such as pantries. Transmission of H. pylori infection could be achieved via inadvertent ingestion of foods contaminated with cockroach excreta containing viable H. pylori.
人类感染幽门螺杆菌的途径仍不明确。在本研究中，我们检测了蟑螂作为幽门螺杆菌可能传播媒介的情况。
我们使用了一种常见的蟑螂品种（黑胸大蠊）。禁食3天后，将蟑螂放置在含有新培养的幽门螺杆菌（悉尼菌株）的琼脂平板上（挑战组）或不含幽门螺杆菌的无菌琼脂平板上（对照组）。挑战24小时后，将蟑螂转移到消毒过的容器中，并提供无菌食物和水。对蟑螂的外表面（腿部和身体）及排泄物进行采样，用于培养、快速尿素酶试验和聚合酶链反应（PCR）。
挑战组蟑螂的排泄物在挑战后24小时可培养出幽门螺杆菌。快速尿素酶试验阳性结果持续到第3天，排泄物中幽门螺杆菌DNA的PCR分析在第7天前均为阳性。相比之下，蟑螂的外表面未培养出幽门螺杆菌。快速尿素酶试验仅在8小时内呈阳性，外表面幽门螺杆菌DNA的PCR分析在1天内呈阳性。
蟑螂通常生活在不卫生的环境中，可能会污染食物和食品储存区域，如食品储藏室。幽门螺杆菌感染可能通过误食被含有活幽门螺杆菌的蟑螂排泄物污染的食物而传播。
