Pressler T, Kronborg G, Shand G H, Mansa B, Høiby N
Department of Paediatrics, Rigshospital, Copenhagen, Denmark.
Med Microbiol Immunol. 1992;181(6):339-49. doi: 10.1007/BF00191546.
IgG subclass antibodies to Pseudomonas aeruginosa outer membrane proteins (OMP) were investigated in serum from cystic fibrosis (CF) patients by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Fifteen patients (eight in good and seven in poor clinical condition) have been followed for an average of 13 years with multiple serum samples covering the preinfection, and early and late stages of chronic infection. Laser-scanning densitometry of photographs taken from immunoblots was used to quantify antibody level and compare with ELISA titres. The earliest anti-OMP antibodies to appear were of the IgG1 subclass. There was no significant difference in IgG subclass antibody levels to OMPs between patients in relatively good and poor clinical condition. Data presented indicate a high positive correlation among measurements of IgG subclass antibodies to P. aeruginosa OMPs using ELISA and immunoblotting.
通过免疫印迹法和酶联免疫吸附测定（ELISA），对囊性纤维化（CF）患者血清中针对铜绿假单胞菌外膜蛋白（OMP）的IgG亚类抗体进行了研究。15名患者（8名临床状况良好，7名临床状况较差）被随访了平均13年，期间采集了多个血清样本，涵盖感染前、慢性感染的早期和晚期阶段。利用免疫印迹照片的激光扫描密度测定法对抗体水平进行定量，并与ELISA滴度进行比较。最早出现的抗OMP抗体属于IgG1亚类。临床状况相对良好和较差的患者之间，针对OMPs的IgG亚类抗体水平没有显著差异。所呈现的数据表明，使用ELISA和免疫印迹法测定针对铜绿假单胞菌OMPs的IgG亚类抗体之间存在高度正相关。
