Morrin M, Reen D J
Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin.
J Med Microbiol. 1993 Dec;39(6):467-72. doi: 10.1099/00222615-39-6-467.
The IgG subclass response to the major outer-membrane proteins (OMPs) of Pseudomonas aeruginosa was investigated in 11 cystic fibrosis (CF) patients and 10 healthy controls. Inhibition of adhesion of P. aeruginosa to buccal epithelial cells by the IgG serum fractions from the CF patients has been established previously. The CF patients demonstrated marked heterogeneity in their individual IgG subclass response to pseudomonal OMPs. The predominant IgG1 and IgG4 responses were directed towards OMPs F, H2 and, with IgG1 only, to protein I. Proteins of 42 and 46 kDa primarily elicited an IgG2 response but some patients produced IgG4 antibodies. The IgG3 response varied from very weak in some patients to a strong reaction with proteins D2, E, G and I in others. The range of antigen-specific IgG subclass responses was similar in CF patients whose IgG fractions strongly inhibited the adherence of P. aeruginosa to epithelial cells and in those whose fractions gave only weak inhibition of adherence. There was no indication that an antibody response towards any particular OMP was implicated in the inhibition of bacterial adherence. Thus, the IgG subclass response to OMPs did not exert a significant effect on adherence when investigated in isolation, but may possibly play some role in combination with other processes.
在11名囊性纤维化（CF）患者和10名健康对照者中研究了对铜绿假单胞菌主要外膜蛋白（OMPs）的IgG亚类反应。此前已证实CF患者血清中的IgG组分可抑制铜绿假单胞菌对颊上皮细胞的黏附。CF患者对假单胞菌OMPs的个体IgG亚类反应表现出明显的异质性。主要的IgG1和IgG4反应针对OMPs F、H2，且IgG1仅针对蛋白I。42 kDa和46 kDa的蛋白主要引发IgG2反应，但部分患者产生IgG4抗体。IgG3反应在一些患者中非常微弱，而在另一些患者中对蛋白D2、E、G和I则有强烈反应。IgG组分强烈抑制铜绿假单胞菌黏附上皮细胞的CF患者与IgG组分仅对黏附有微弱抑制作用的患者相比，其抗原特异性IgG亚类反应范围相似。没有迹象表明针对任何特定OMP的抗体反应与细菌黏附的抑制有关。因此，单独研究时，对OMPs的IgG亚类反应对黏附没有显著影响，但可能与其他过程共同发挥某种作用。
