Villanueva Iván, Piñón Manuel, Quevedo-Corona Lucía, Martínez-Olivares Rubén, Racotta Radu
Departamento de Fisiología, Escuela Nacional de Ciencias Biológicas, Col. Casco de Sto. Tomás, México.
Life Sci. 2003 Aug 15;73(13):1645-53. doi: 10.1016/s0024-3205(03)00491-0.
Chemical sympathectomy with guanethidine (Gnt) selectively destroys the postganglionic noradrenergic neurons, whereas dopaminergic fibers and nonneural catecholamine-secreting cells are spared. As a result, the relative proportions of norepinephrine (NE), epinephrine (E), and dopamine (DA) in tissues can be differentially affected. This study was done to show the possible differences in the relative amount of catecholamines in some organs and tissues that might indicate the nature of the secretory cells from which they originate. The contents of NE, E, and DA were assessed in rats neonatally treated with Gnt. Gnt-treated rats showed significantly lower levels of NE (P < 0.01) in all tissues except the adrenal gland and paraganglia. Epinephrine was present in all tissues with mean levels below 25 ng/g, with the exception of the adrenal gland (700 microg/gland) and paraganglia (100 ng/g). Only the heart showed lower values in Gnt-treated rats. Mean DA levels were also very high in paraganglia (530 ng/g). In the Gnt-treated rats, DA levels fell practically to zero except in the duodenum, mesentery, and adrenal, whereas there were high levels in the paraganglia, which were significantly different from controls. The results suggest that the three catecholamines are contained mainly in noradrenergic sympathetic fibers of muscle, white adipose tissue, heart, liver, pancreas, and spleen. The duodenum and mesentery may have dopaminergic fibers or E- and DA-containing nonneural cells. Hepatic-vagus paraganglia contain all the catecholamines in relatively high amounts in nonneural cells, and Gnt treatment raises DA levels without affecting the other amines.
用胍乙啶（Gnt）进行化学性交感神经切除术可选择性地破坏节后去甲肾上腺素能神经元，而多巴胺能纤维和非神经儿茶酚胺分泌细胞则不受影响。因此，组织中去甲肾上腺素（NE）、肾上腺素（E）和多巴胺（DA）的相对比例可能会受到不同影响。本研究旨在表明某些器官和组织中儿茶酚胺相对含量的可能差异，这些差异可能表明它们所源自的分泌细胞的性质。对新生期用Gnt处理的大鼠的NE、E和DA含量进行了评估。Gnt处理的大鼠除肾上腺和副神经节外，所有组织中的NE水平均显著降低（P < 0.01）。除肾上腺（700μg/腺体）和副神经节（100ng/g）外，所有组织中均存在肾上腺素，其平均水平低于25ng/g。只有心脏在Gnt处理的大鼠中显示出较低的值。副神经节中的平均DA水平也非常高（530ng/g）。在Gnt处理的大鼠中，除十二指肠、肠系膜和肾上腺外，DA水平几乎降至零，而副神经节中的DA水平较高，与对照组有显著差异。结果表明，这三种儿茶酚胺主要存在于肌肉、白色脂肪组织、心脏、肝脏、胰腺和脾脏的去甲肾上腺素能交感纤维中。十二指肠和肠系膜可能含有多巴胺能纤维或含E和DA的非神经细胞。肝迷走神经副神经节的非神经细胞中含有相对较高含量的所有儿茶酚胺，Gnt处理可提高DA水平而不影响其他胺类。
