Pérez-Juárez Angélica, Aguirre-Pérez Andrea Giovanna, Barrientos-Alvarado Cornelio
Departament of Physiology, Higher School of Medicine, National Polytechnic Institute, Mexico City 11340, Mexico.
Faculty of Science and Mathematics, Arkansas State University, Queretaro City 75270, Mexico.
Life (Basel). 2022 Dec 19;12(12):2147. doi: 10.3390/life12122147.
Diabetes mellitus (DM) is a metabolic disorder whose prevalence has continuously increased worldwide and is associated with dysfunction of the autonomic nervous system and, in particular, that of the sympathetic nervous system (SNS). The objective of this study was to analyze the interaction of DM and the SNS, building a model of sympathectomized diabetic rats to determine alterations in the content of CA (catecholamines) in different intra-abdominal organs. Sympathectomy was conducted with guanethidine (GNT). Additionally, DM was induced with STZ (Streptozotocin). Treatment with GNT decreased norepinephrine (NE) content in all analyzed tissues, with significant differences found in the paraganglia, liver, pancreas, duodenum, and heart compared to the control group. With respect to epinephrine (E), which was only found in the liver, pancreas, and heart, presenting significant differences (p < 0.05) in the heart, a decrease in its concentration was observed for all of the experimental groups with respect to the control. The decrease in dopamine (DA) content due to the GNT−STZ treatment was 30.1% in the heart with respect to the diabetic (STZ) group. The amount of CA in the adrenal medulla indicates the effect of sympathectomy on the GNT group where there was a significant reduction (p < 0.05) of DA. These findings suggest that the elimination of the sympathetic nervous system in diabetic organisms contributed to a decrease in blood glucose; likewise, an alteration in the levels of CA was observed in the different selected organs, possibly attributed to the severity, duration, and pathogenesis of the complications of acute and chronic DM.
糖尿病（DM）是一种代谢紊乱疾病，其在全球范围内的患病率持续上升，并且与自主神经系统功能障碍相关，尤其是交感神经系统（SNS）功能障碍。本研究的目的是分析糖尿病与交感神经系统的相互作用，构建去交感神经的糖尿病大鼠模型，以确定不同腹腔内器官中儿茶酚胺（CA）含量的变化。用胍乙啶（GNT）进行去交感神经手术。此外，用链脲佐菌素（STZ）诱导糖尿病。GNT治疗降低了所有分析组织中的去甲肾上腺素（NE）含量，与对照组相比，在副神经节、肝脏、胰腺、十二指肠和心脏中发现有显著差异。关于仅在肝脏、胰腺和心脏中发现的肾上腺素（E），在心脏中呈现出显著差异（p<0.05），所有实验组相对于对照组其浓度均下降。与糖尿病（STZ）组相比，GNT-STZ治疗导致心脏中多巴胺（DA）含量下降30.1%。肾上腺髓质中CA的含量表明去交感神经手术对GNT组的影响，其中DA有显著降低（p<0.05）。这些发现表明，消除糖尿病生物体中的交感神经系统有助于降低血糖；同样，在不同选定器官中观察到CA水平的改变，这可能归因于急性和慢性糖尿病并发症的严重程度、持续时间和发病机制。
