Xie Yong, Inoue Tsuyoshi, Miyamoto Youichi, Matsumura Hiroyoshi, Kataoka Kunishige, Yamaguchi Kazuya, Suzuki Shinnichiro, Kai Yasushi
Department of Materials Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1474-6. doi: 10.1107/s0907444903011326. Epub 2003 Jul 23.
Mavicyanin isolated from Cucurbita pepo medullosa is a glycosylated protein containing a single polypeptide chain of 109 amino-acid residues and is a member of the phytocyanin subclass of cupredoxins. Non-glycosylated recombinant mavicyanin, which was expressed in Escherichia coli, was crystallized by the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant at pH 5.5. The crystals belonged to the hexagonal space group P6(1) (or P6(5)), with unit-cell parameters a = 64.0, c = 245.0 A, four molecules per asymmetric unit and a solvent content of 59%. X-ray diffraction data were collected to 1.6 A resolution. To solve the structure of mavicyanin, the MAD method as well as a Patterson search method using the structure of stellacyanin as a starting model are presently being utilized.
从西葫芦髓质中分离出的马维花青素是一种糖基化蛋白，含有一条由109个氨基酸残基组成的单多肽链，属于铜蓝蛋白中植物花青素亚类的成员。在大肠杆菌中表达的非糖基化重组马维花青素，通过悬滴气相扩散法，以硫酸铵作为沉淀剂，在pH 5.5条件下结晶。晶体属于六方空间群P6(1)（或P6(5)），晶胞参数a = 64.0，c = 245.0 Å，每个不对称单元有四个分子，溶剂含量为59%。X射线衍射数据收集到1.6 Å分辨率。为了解决马维花青素的结构，目前正在使用MAD方法以及以星蓝蛋白结构为起始模型的帕特森搜索方法。
