Lennon Aine M
Department of Operative Dentistry, Preventive Dentistry and Periodontology, Georg-August-University Göttingen, Göttingen, Germany.
Oper Dent. 2003 Jul-Aug;28(4):341-5.
A recent study showed that orange-red fluorescence in carious dentin could be used to detect residual caries (Lennon & others, 2002). This study compared the ability of a new fluorescence-aided caries excavation technique (FACE) with the conventional method. Forty extracted teeth with occlusal dentin caries were selected. The teeth were bisected longitudinally through the center of the lesion. Lesion depth and width were measured and the teeth were divided into two groups of 20, each with the same average lesion size. The tooth halves were reassembled and fixed by embedding the roots in acrylic resin. Access cavities were prepared using a high-speed handpiece and diamond fissure bur. In the FACE group, violet light (370-420 nm) was fed into the fiber optics of a slow-speed hand-piece, so that it illuminated the operating field. The cavity was observed through a 530-nm high-pass filter and orange-red fluorescing areas were removed. In the conventional group, a sharp probe was used to detect soft dentin, which was removed. One-half of each tooth was stained for bacteria using Ethidium Bromide and examined using Confocal Laser Scanning Microscopy (CLSM). Bacteria were present in significantly (p=0.037) fewer FACE samples (3) compared to conventional samples (9). It can be concluded that the new method is more effective than conventional caries excavation.
最近的一项研究表明，龋损牙本质中的橙红色荧光可用于检测残余龋（列侬等人，2002年）。本研究比较了一种新的荧光辅助龋洞制备技术（FACE）与传统方法的效能。选取了40颗患有咬合面牙本质龋的离体牙。沿病变中心将牙齿纵向一分为二。测量病变深度和宽度，并将牙齿分为两组，每组20颗，两组平均病变大小相同。将牙齿的两半重新组装，通过将牙根嵌入丙烯酸树脂进行固定。使用高速手机和金刚石裂钻制备进入洞。在FACE组中，将紫光（370 - 420纳米）导入低速手机的光纤中，使其照亮术野。通过530纳米的高通滤光片观察龋洞，去除橙红色荧光区域。在传统组中，使用锐利探针检测软牙本质并予以去除。每颗牙齿的一半用溴化乙锭对细菌进行染色，并用共聚焦激光扫描显微镜（CLSM）检查。与传统样本（9个）相比，FACE样本中存在细菌的数量显著更少（p = 0.037）（3个）。可以得出结论，新方法比传统龋洞制备方法更有效。
