Tom Henry, Chan Kenneth H, Darling Cynthia L, Fried Daniel
Department of Preventive and Restorative Dental Science, University of California, San Francisco, California, 94143-0758.
Lasers Surg Med. 2016 Jan;48(1):52-61. doi: 10.1002/lsm.22438. Epub 2016 Jan 13.
Studies have shown that reflectance images at near-IR wavelengths coincident with higher water absorption are well-suited for image-guided laser ablation of carious lesions since the contrast between sound and demineralized enamel is extremely high and interference from stains is minimized. The objective of this study was to demonstrate that near-IR reflectance images taken at a wavelength range of 1,500-1,700 nm can be used to guide a 9.3 μm CO(2) laser for the selective ablation of early demineralization on tooth occlusal surfaces.
The occlusal surfaces of ten sound human molars were used in this in vitro study. Shallow simulated caries lesions with random patterns and varying depth and position were produced on tooth occlusal surfaces. Sequential near-IR reflectance images at 1,500-1,700 nm were used to guide the laser for the selective removal of the demineralized enamel. Digital microscopy and polarization sensitive optical coherence tomography (PS-OCT) were used to assess selectivity.
Images taken before and after lesion removal suggest that the demineralized areas were removed with high selectivity. Although the estimated volume of tissue ablated was typically higher than the initial lesion volume measured with PS-OCT, the volume of enamel removed by the laser correlated well with the initial lesion volume.
Sequential near-IR reflectance images at 1,500-1,700 nm can be used to guide a 9.3 μm CO(2) laser for the selective ablation of early demineralization on tooth occlusal surfaces.
研究表明,与较高水吸收率一致的近红外波长反射图像非常适合用于龋齿病变的图像引导激光消融,因为健全牙釉质与脱矿牙釉质之间的对比度极高,且污渍的干扰降至最低。本研究的目的是证明在1500 - 1700纳米波长范围内拍摄的近红外反射图像可用于引导9.3微米二氧化碳激光,以选择性消融牙齿咬合面上的早期脱矿区域。
本体外研究使用了十颗健全人类磨牙的咬合面。在牙齿咬合面上制作了具有随机图案、不同深度和位置的浅模拟龋损。使用1500 - 1700纳米的连续近红外反射图像引导激光选择性去除脱矿牙釉质。使用数字显微镜和偏振敏感光学相干断层扫描(PS - OCT)评估选择性。
病变去除前后拍摄的图像表明,脱矿区域被高选择性地去除。尽管估计消融的组织体积通常高于用PS - OCT测量的初始病变体积,但激光去除的牙釉质体积与初始病变体积相关性良好。
1500 - 1700纳米的连续近红外反射图像可用于引导9.3微米二氧化碳激光选择性消融牙齿咬合面上的早期脱矿区域。