Rudd Stephen
Institut für Bioinformatik, GSF Forschungszentrum für Umwelt und Gesundheit, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany.
Trends Plant Sci. 2003 Jul;8(7):321-9. doi: 10.1016/S1360-1385(03)00131-6.
Over three million sequences from approximately 200 plant species have been deposited in the publicly available plant expressed sequence tag (EST) sequence databases. Many of the ESTs have been sequenced as an alternative to complete genome sequencing or as a substrate for cDNA array-based expression analyses. This creates a formidable resource from both biodiversity and gene-discovery standpoints. Bioinformatics-based sequence analysis tools have extended the scope of EST analysis into the fields of proteomics, marker development and genome annotation. Although EST collections are certainly no substitute for a whole genome scaffold, this "poor man's genome" resource forms the core foundations for various genome-scale experiments within the as yet unsequenceable plant genomes.
来自约200种植物物种的超过300万个序列已存入可公开获取的植物表达序列标签（EST）序列数据库。许多EST已被测序，作为全基因组测序的替代方法，或作为基于cDNA阵列的表达分析的底物。从生物多样性和基因发现的角度来看，这创造了一个强大的资源。基于生物信息学的序列分析工具已将EST分析的范围扩展到蛋白质组学、标记开发和基因组注释领域。虽然EST文库肯定不能替代完整的基因组框架，但这种“穷人的基因组”资源构成了尚未测序的植物基因组内各种基因组规模实验的核心基础。
