Assimacopoulos Stavroula, Grove Elizabeth A, Ragsdale Clifton W
Department of Neurobiology, Pharmacology, and Physiology, The University of Chicago, Chicago, Illinois 60637, USA.
J Neurosci. 2003 Jul 23;23(16):6399-403. doi: 10.1523/JNEUROSCI.23-16-06399.2003.
In an emerging model, area patterning of the mammalian cerebral cortex is regulated in part by embryonic signaling centers. Two have been identified: an anterior telencephalic source of fibroblast growth factors and the cortical hem, a medial structure expressing winglessint (WNT) and bone morphogenetic proteins. We describe a third signaling source, positioned as a mirror image of the cortical hem, along the lateral margin of the cortical primordium. The cortical antihem is identified by gene expression for three epidermal growth factor (EGF) family members, Tgf(alpha), Neuregulin 1, and Neuregulin 3, as well as two other signaling molecules, Fgf7 and the secreted WNT antagonist Sfrp2. We find that the antihem is lost in mice homozygous for the Small eye (Pax6) mutation and suggest the loss of EGF signaling at least partially explains defects in cortical patterning and cell migration in Small eye mice.
在一种新出现的模型中，哺乳动物大脑皮层的区域模式部分受胚胎信号中心调控。已确定两个信号中心：一个是成纤维细胞生长因子的前脑来源，另一个是皮质下托，它是一个表达无翅型整合（WNT）和骨形态发生蛋白的内侧结构。我们描述了第三个信号源，它沿着皮质原基的外侧边缘定位，与皮质下托呈镜像对称。通过三种表皮生长因子（EGF）家族成员——转化生长因子α（Tgfα）、神经调节蛋白1和神经调节蛋白3，以及另外两种信号分子——成纤维细胞生长因子7（Fgf7）和分泌型WNT拮抗剂分泌型卷曲相关蛋白2（Sfrp2）的基因表达来确定皮质抗下托。我们发现小眼（Pax6）突变纯合小鼠缺失抗下托，并表明EGF信号缺失至少部分解释了小眼小鼠皮质模式和细胞迁移的缺陷。
