Dahlem Yuliya A, Dahlem Markus A, Mair Thomas, Braun Katharina, Müller Stefan C
Institut für Experimentelle Physik, Abteilung Biophysik, Otto-von-Guericke-Universität Magdeburg, Universitätsplatz 2, 39016 Magdeburg, Germany.
Exp Brain Res. 2003 Sep;152(2):221-8. doi: 10.1007/s00221-003-1545-y. Epub 2003 Jul 23.
The phenomenon of spreading depression (SD) was observed in chicken retina by means of optical registration via a microscope and a CCD camera applying modern methods of image processing for optimized evaluation of the wave profiles. The propagation dynamics of SD waves was investigated as a function of extracellular potassium. Two main findings were obtained. Firstly, the frequency of spontaneous wave generation increased with the increase of K+ concentration. Secondly, there was an effect of potassium on the wave profile. In particular, the recovery zone of SD waves was shortened at increased K+. This effect was not only due to the dispersion relation of waves in excitable media as shown by the result of the mechanically induced wave trains. Applying the basic principles of chemical excitability for the interpretation of the data led us to the conclusion that these potassium effects are due to perturbations of an autocatalytic reaction to be further explored.
通过显微镜和电荷耦合器件（CCD）相机进行光学记录，并应用现代图像处理方法对波剖面进行优化评估，在鸡视网膜中观察到了扩散性抑制（SD）现象。研究了SD波的传播动力学与细胞外钾的关系。获得了两个主要发现。首先，自发波产生的频率随着K⁺浓度的增加而增加。其次，钾对波剖面有影响。特别是，在K⁺浓度增加时，SD波的恢复区缩短。这种效应不仅如机械诱导波列的结果所示，是由于可兴奋介质中波的色散关系。应用化学兴奋性的基本原理来解释数据，使我们得出结论，这些钾效应是由于自催化反应的扰动，有待进一步探索。
