Wiedemann M, Hanke W
Institute of Physiology, University of Hoheneheim, Stuttgart, Germany.
Neurosci Lett. 1997 Aug 29;232(2):99-102. doi: 10.1016/s0304-3940(97)00578-8.
The aim of this study was to establish an experimental model to investigate neuronal lesions. The retina is an easy accessible model system to study central nervous system (CNS) disorders or neuronal effective drugs. It consists of only a few characteristic layers and is easy to prepare as an intact piece of tissue. In the retina the typical cell swelling of a developing lesion is accompanied by a very strong intrinsic optical signal (IOS) which is simultaneous with the electrical signal and is based on changes in light scattering. The IOS can be easily observed during the whole experiment and can be recorded with non-invasive optical methods for further quantification of damage. In the developed model, the lesions are elicited electrically with a tungsten microelectrode (0.1 M[omega]). The degree of damage depends on the magnitude of the stimulus. The parameters for the quantification of damage are the area and the brightness of the affected tissue. The growth of the lesions can be influenced with drugs added to the perfusion system. In the present study we tested, glycerol, mannitol and ketamine which are known to be neuroprotective in other animal models.
本研究的目的是建立一个用于研究神经元损伤的实验模型。视网膜是一个易于获取的模型系统，可用于研究中枢神经系统（CNS）疾病或神经元有效药物。它仅由少数几个特征层组成，并且易于制备成完整的组织块。在视网膜中，发育性损伤的典型细胞肿胀伴随着非常强烈的内在光学信号（IOS），该信号与电信号同时出现，并且基于光散射的变化。在整个实验过程中可以很容易地观察到IOS，并且可以用非侵入性光学方法进行记录，以进一步量化损伤。在已建立的模型中，使用钨微电极（0.1 MΩ）通过电刺激引发损伤。损伤程度取决于刺激的强度。损伤量化的参数是受影响组织的面积和亮度。损伤的发展可以通过添加到灌注系统中的药物来影响。在本研究中，我们测试了甘油、甘露醇和氯胺酮，已知它们在其他动物模型中具有神经保护作用。
