Coulibaly I, Revol B, Noirot M, Poncet V, Lorieux M, Carasco-Lacombe C, Minier J, Dufour M, Hamon P
IRD, UMR 1097, 911 av Agropolis, BP 64501, 34394 Montpellier cedex 5, France.
Theor Appl Genet. 2003 Oct;107(6):1148-55. doi: 10.1007/s00122-003-1355-4. Epub 2003 Jul 23.
An interspecific cross (BC 1) involving a species with one of the largest genomes in the Coffea genus [ Coffea heterocalyx (HET), qDNA = 1.74 pg] and a species with a medium-sized genome [ Coffea canephora (CAN), qDNA = 1.43 pg] was studied using two types of molecular markers, AFLP and SSR. One hundred and eighty eight AFLP bands and 34 SSR primer pairs were suitable for mapping. The total map length was 1,360 cM with 190 loci distributed in 15 linkage groups. The results were compared to those obtained previously on an interspecific BC 1 progeny involving a species with a medium-sized genome ( Coffea liberica var dewevrei, DEW) and a species with one of the smallest genomes ( Coffea pseudozanguebariae, PSE). They are discussed relative to three main points: (1) the relevance of the different marker types, (2) the genomic distribution of AFLP and SSR markers, and (3) the relation between AFLP polymorphism and genome size.
利用AFLP和SSR两种分子标记，对涉及咖啡属中具有最大基因组之一的物种[异色咖啡（HET），qDNA = 1.74 pg]和具有中等大小基因组的物种[卡内弗拉咖啡（CAN），qDNA = 1.43 pg]的种间杂交（BC1）进行了研究。188条AFLP条带和34对SSR引物对适用于作图。总图长为1360 cM，190个位点分布在15个连锁群中。将这些结果与之前在涉及中等大小基因组物种（利比里卡变种德韦夫雷咖啡，DEW）和最小基因组物种之一（拟桑给巴尔咖啡，PSE）的种间BC1后代上获得的结果进行了比较。从三个主要方面进行了讨论：（1）不同标记类型的相关性，（2）AFLP和SSR标记的基因组分布，以及（3）AFLP多态性与基因组大小之间的关系。
