Poncet V, Hamon P, de Saint Marc M-B Sauvage, Bernard T, Hamon S, Noirot M
UMR 1097 Diversité et Génome des Plantes Cultivées, Centre IRD, 911 avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France.
J Hered. 2005 Jan-Feb;96(1):59-65. doi: 10.1093/jhered/esi013. Epub 2004 Dec 15.
Amplified fragment length polymorphism (AFLP) is often used for genetic mapping and diversity analysis, but very little information is currently available on their sequence characteristics. Species-specific sequences were analyzed from a single Coffea genome (Coffea pseudozanguebariae) associated with clustered or nonclustered AFLP loci of known genetic position. Compared with the expressed sequence tag (EST) sequence composition, their AT content exhibited a bimodal distribution with AT-poor sequences corresponding mainly to putative coding sequences. AT-rich sequences, apart from the EST distribution, were usually clustered on the genetic map and might correspond to noncoding sequences. Conversion of these AFLP markers into sequence-characterized amplified region (SCAR) anchor markers allowed us to assess sequence conservation within Coffea species with respect to species relatedness.
扩增片段长度多态性（AFLP）常用于遗传图谱构建和多样性分析，但目前关于其序列特征的信息非常少。从一个与已知遗传位置的成簇或非成簇AFLP位点相关的单一咖啡基因组（拟桑给巴尔咖啡）中分析了物种特异性序列。与表达序列标签（EST）序列组成相比，它们的AT含量呈现双峰分布，富含AT的序列主要对应于推定的编码序列。除了EST分布外，富含AT的序列通常聚集在遗传图谱上，可能对应于非编码序列。将这些AFLP标记转化为序列特征扩增区域（SCAR）锚定标记，使我们能够根据物种亲缘关系评估咖啡属物种内的序列保守性。
