Lübbehüsen T L, Nielsen J, McIntyre M
Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, 2800 Kgs. Lyngby, Denmark.
Appl Microbiol Biotechnol. 2004 Feb;63(5):543-8. doi: 10.1007/s00253-003-1394-4. Epub 2003 Jul 16.
The dimorphic organism Mucor circinelloides is currently being investigated as a potential host for heterologous protein production. The production of ethanol on pentose and hexose sugars was studied in submerged batch cultivations to further the general knowledge of Mucor physiology, with a view to the minimisation or elimination of the by-product ethanol for future process design. Large amounts of ethanol were produced during aerobic growth on glucose under non-oxygen limiting conditions, which is indicative of M. circinelloides being a Crabtree-positive organism. Ethanol production on galactose or xylose was less significant. The response of the organism to increased ethanol concentrations, both as the sole carbon source and in the presence of a sugar, was investigated in terms of biomass formation and morphology.
双态生物卷枝毛霉目前正作为异源蛋白生产的潜在宿主进行研究。在分批深层培养中研究了戊糖和己糖上乙醇的产生情况，以进一步了解毛霉生理学的一般知识，目的是在未来的工艺设计中尽量减少或消除副产物乙醇。在非氧气限制条件下，在葡萄糖上进行有氧生长时会产生大量乙醇，这表明卷枝毛霉是一种克奈特阳性生物。在半乳糖或木糖上乙醇的产生则不太显著。研究了该生物对乙醇浓度增加的反应，包括作为唯一碳源以及在有糖存在的情况下，从生物量形成和形态方面进行了研究。
