Kovács G M, Vágvölgyi C, Oberwinkler F
Department of Botany, University of Szeged, 6701 Szeged, Hungary.
Folia Microbiol (Praha). 2003;48(3):369-78. doi: 10.1007/BF02931369.
The type of the in vitro root interactions of Terfezia terfezioides with the plants Robinia pseudoacacia and Helianthemum ovatum was investigated including detailed anatomical and ultrastructural characterization. No difference in growth was detected at different phosphate concentrations on agar synthetic medium between the inoculated and control plants during a short-time cultivation. The fungal colonization of the roots increased with higher phosphate level in both plant species, but was always lower in R. pseudoacacia roots. Septate hyphae formed frequently intracellular branched coils in dead cortical cells. In H. ovatum intercellular hyphae were observed forming finger-like structures reminiscent of Hartig-net structures in ectomycorrhizae. A loose hyphal envelope covered the root surface of both colonized and noncolonized roots. The features resembled similar structures described earlier during the mycorrhizae of different Terfezia species. Our detailed anatomical and ultrastructural study shows that the in vitro root interactions of the T. terfezioides cannot be considered unambiguously as mycorrhiza.
研究了块菌与刺槐和椭圆叶半日花在体外根系的相互作用类型，包括详细的解剖学和超微结构特征。在短期培养期间，接种和对照植物在琼脂合成培养基上不同磷酸盐浓度下未检测到生长差异。两种植物根系的真菌定殖都随着磷酸盐水平的升高而增加，但在刺槐根系中始终较低。有隔菌丝在死亡的皮层细胞中频繁形成细胞内分支线圈。在椭圆叶半日花中，观察到细胞间菌丝形成指状结构，类似于外生菌根中的哈蒂氏网结构。一个松散的菌丝包膜覆盖了定殖和未定殖根系的根表面。这些特征类似于早期描述的不同块菌物种菌根中的类似结构。我们详细的解剖学和超微结构研究表明，块菌的体外根系相互作用不能明确地被视为菌根。
